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Literature summary extracted from

  • Kochhar, S.; Chuard, N.; Hottinger, H.
    Glutamate 264 modulates the pH dependence of the NAD(+)-dependent D-lactate dehydrogenase (1992), J. Biol. Chem., 267, 20298-20301.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.28 expression in Escherichia coli Lactobacillus delbrueckii subsp. bulgaricus

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.28 E264G shift of 2 units in optimal pH toward the acidic range Lactobacillus delbrueckii subsp. bulgaricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.28 1.5
-
 pyruvate
-
 Lactobacillus delbrueckii subsp. bulgaricus
1.1.1.28 7
-
 oxaloacetate
-
 Lactobacillus delbrueckii subsp. bulgaricus
1.1.1.28 10
-
 Bromopyruvate
-
 Lactobacillus delbrueckii subsp. bulgaricus
1.1.1.28 100
-
 glyoxylate
-
 Lactobacillus delbrueckii subsp. bulgaricus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.28 (R)-lactate + NAD+ Lactobacillus delbrueckii subsp. bulgaricus
-
 pyruvate + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.28 Lactobacillus delbrueckii subsp. bulgaricus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.28
-
 Lactobacillus delbrueckii subsp. bulgaricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.28 (R)-lactate + NAD+
-
 Lactobacillus delbrueckii subsp. bulgaricus pyruvate + NADH
-
 ?
1.1.1.28 bromopyruvate + NADH
-
 Lactobacillus delbrueckii subsp. bulgaricus 3-bromo-2-hydroxypropanoate + NAD+
-
 ?
1.1.1.28 glyoxylate + NADH
-
 Lactobacillus delbrueckii subsp. bulgaricus hydroxyacetic acid + NAD+
-
 ?
1.1.1.28 oxaloacetate + NADH
-
 Lactobacillus delbrueckii subsp. bulgaricus malate + NAD+
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.28 7.5
-
-
 Lactobacillus delbrueckii subsp. bulgaricus