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Literature summary extracted from

  • Schlesinger, M.J.; Coon, M.J.
    Reduction of mevaldic acid to mevalonic acid by a partially purified enzyme from liver (1961), J. Biol. Chem., 236, 2421-2424.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.32 (R,S)-mevaldate 20 mM or higher Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.32 0.003
-
 NADH
-
 Sus scrofa
1.1.1.32 0.09
-
 NADPH
-
 Sus scrofa
1.1.1.32 4
-
 mevaldate
-
 Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.32 (R)-3-hydroxy-3-methyl-5-oxovaleric acid + NADH Sus scrofa
-
 (R)-3-hydroxy-3-methyl-5-hydroxyvaleric acid + NAD+
-
 ir
1.1.1.32 (S)-3-hydroxy-3-methyl-5-oxovaleric acid + NADH Sus scrofa
-
 (S)-3-hydroxy-3-methyl-5-hydroxyvaleric acid + NAD+
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.32 Rattus norvegicus
-
-
-
1.1.1.32 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.32 ammonium sulfate precipitation, calcium phosphate gel, DEAE-cellulose, bentonite precipitation Sus scrofa

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.32 (R)-mevalonate + NAD+ = mevaldate + NADH + H+ formation of mevalonic acid is strongly favoured Sus scrofa
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.32 liver
-
 Rattus norvegicus
-
1.1.1.32 liver
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.32 0.63
-
 partially purified liver enzyme Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.32 (R)-3-hydroxy-3-methyl-5-oxovaleric acid + NADH
-
 Sus scrofa (R)-3-hydroxy-3-methyl-5-hydroxyvaleric acid + NAD+
-
 ir
1.1.1.32 (R)-3-hydroxy-3-methyl-5-oxovaleric acid + NADPH
-
 Sus scrofa (R)-3-hydroxy-3-methyl-5-hydroxyvaleric acid + NADP+
-
 ir
1.1.1.32 (S)-3-hydroxy-3-methyl-5-oxovaleric acid + NADH
-
 Sus scrofa (S)-3-hydroxy-3-methyl-5-hydroxyvaleric acid + NAD+
-
 ir
1.1.1.32 mevaldate + NADH + H+
-
 Sus scrofa (R)-mevalonate + NAD+
-
 r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.32 5.4
-
-
 Sus scrofa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.32 4.6 6.9
-
 Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.32 NADH
-
 Sus scrofa
1.1.1.32 NADPH
-
 Rattus norvegicus