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Literature summary extracted from

  • Bochar, D.A.; Brown, J.R.; Doolittle, W.F.; Klenk, H.P.; Lam, W.; Schenk, M.E.; Stauffacher, C.V.; Rodwell, V.W.
    3-Hydroxy-3-methylglutaryl coenzyme A reductase of Sulfolobus solfataricus: DNA sequence, phylogeny, expression in Escherichia coli of the hmgA gene, and purification and kinetic properties of the gene product (1997), J. Bacteriol., 179, 3632-3638.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.34 expression in Escherichia coli Saccharolobus solfataricus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.34 lovastatin competitive inhibitor for HMG-CoA binding site Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.34 0.017
-
3-hydroxy-3-methylglutaryl-CoA
-
Saccharolobus solfataricus
1.1.1.34 0.017
-
3-hydroxy-3-methylglutaryl-CoA pH 5.5, 50°C Saccharolobus solfataricus
1.1.1.34 0.023
-
NADPH
-
Saccharolobus solfataricus
1.1.1.34 0.023
-
NADPH pH 5.5, 50°C Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.34 3-hydroxy-3-methylglutaryl-CoA + NADPH Saccharolobus solfataricus
-
(R)-mevalonate + CoA + NADP+
-
?
1.1.1.34 3-hydroxy-3-methylglutaryl-CoA + NADPH Saccharolobus solfataricus P2
-
(R)-mevalonate + CoA + NADP+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.34 Saccharolobus solfataricus Q980N1
-
-
1.1.1.34 Saccharolobus solfataricus P2 Q980N1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.34
-
Saccharolobus solfataricus
1.1.1.34 recombinant enzyme Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.34 17.5
-
50°C, pH 5.5, recombinant enzyme Saccharolobus solfataricus
1.1.1.34 17500
-
-
Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.34 3-hydroxy-3-methylglutaryl-CoA + NADPH
-
Saccharolobus solfataricus (R)-mevalonate + CoA + NADP+
-
?
1.1.1.34 3-hydroxy-3-methylglutaryl-CoA + NADPH substrate is (S)-isomer of HMG-CoA Saccharolobus solfataricus (R)-mevalonate + CoA + NADP+
-
?
1.1.1.34 3-hydroxy-3-methylglutaryl-CoA + NADPH
-
Saccharolobus solfataricus P2 (R)-mevalonate + CoA + NADP+
-
?
1.1.1.34 3-hydroxy-3-methylglutaryl-CoA + NADPH substrate is (S)-isomer of HMG-CoA Saccharolobus solfataricus P2 (R)-mevalonate + CoA + NADP+
-
?
1.1.1.34 additional information no substrate: NADH Saccharolobus solfataricus ?
-
?
1.1.1.34 additional information no substrate: NADH Saccharolobus solfataricus P2 ?
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.34 85
-
-
Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.34 90
-
stable Saccharolobus solfataricus
1.1.1.34 90
-
half-life at 90°C is 3.2 h Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.34 5.5
-
-
Saccharolobus solfataricus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.34 NADPH
-
Saccharolobus solfataricus