EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.36 | alpha-ketoglutarate | 20% activation | Methylorubrum extorquens | |
1.1.1.36 | ATP | 32% activation | Methylorubrum extorquens | |
1.1.1.36 | citrate | 30% activation | Methylorubrum extorquens | |
1.1.1.36 | isocitrate | 25% activation, stimulates NADP+ regeneration | Methylorubrum extorquens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.36 | acetoacetyl-CoA | - |
Azotobacter vinelandii | |
1.1.1.36 | acetoacetyl-CoA | - |
Cupriavidus necator | |
1.1.1.36 | acetoacetyl-CoA | concentration above 40 mM | Methylorubrum extorquens | |
1.1.1.36 | acetoacetyl-CoA | - |
Methylorubrum rhodesianum | |
1.1.1.36 | acetoacetyl-CoA | - |
Zoogloea ramigera | |
1.1.1.36 | NAD+ | 17% inhibition | Methylorubrum extorquens | |
1.1.1.36 | NAD+ | - |
Methylorubrum rhodesianum | |
1.1.1.36 | NADP+ | 40% inhibition | Methylorubrum extorquens | |
1.1.1.36 | NADP+ | - |
Methylorubrum rhodesianum | |
1.1.1.36 | NADPH | - |
Azotobacter vinelandii | |
1.1.1.36 | NADPH | concentrationgreater 100 mM | Methylorubrum extorquens | |
1.1.1.36 | NADPH | - |
Methylorubrum rhodesianum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.36 | 0.005 | - |
acetoacetyl-CoA | - |
Cupriavidus necator | |
1.1.1.36 | 0.011 | - |
acetoacetyl-CoA | 100 mM NADPH | Azotobacter vinelandii | |
1.1.1.36 | 0.0116 | - |
acetoacetyl-CoA | - |
Methylorubrum extorquens | |
1.1.1.36 | 0.015 | - |
acetoacetyl-CoA | - |
Methylorubrum rhodesianum | |
1.1.1.36 | 0.018 | - |
NADPH | - |
Methylorubrum rhodesianum | |
1.1.1.36 | 0.019 | - |
NADPH | - |
Cupriavidus necator | |
1.1.1.36 | 0.02 | - |
NADPH | 32 mM acetoacetyl-CoA | Azotobacter vinelandii | |
1.1.1.36 | 0.03 | - |
D-beta-hydroxybutyryl-CoA | - |
Methylorubrum rhodesianum | |
1.1.1.36 | 0.031 | - |
NADP+ | - |
Cupriavidus necator | |
1.1.1.36 | 0.033 | - |
D-hydroxybutyryl-CoA | - |
Cupriavidus necator | |
1.1.1.36 | 0.041 | - |
NADPH | - |
Methylorubrum extorquens | |
1.1.1.36 | 0.06 | - |
NADP+ | - |
Methylorubrum rhodesianum | |
1.1.1.36 | 0.25 | - |
NAD+ | - |
Cupriavidus necator | |
1.1.1.36 | 0.4 | - |
NADH | - |
Cupriavidus necator |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.1.36 | soluble | - |
Zoogloea ramigera | - |
- |
1.1.1.36 | soluble | - |
Methylorubrum extorquens | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.36 | 31000 | - |
4 * 31000, 12% PAGE | Methylorubrum extorquens |
1.1.1.36 | 77000 | - |
- |
Azotobacter vinelandii |
1.1.1.36 | 84000 | - |
- |
Cupriavidus necator |
1.1.1.36 | 92000 | - |
- |
Zoogloea ramigera |
1.1.1.36 | 140000 | - |
gel-filtration | Methylorubrum extorquens |
1.1.1.36 | 141000 | - |
gradient PAGE | Methylorubrum extorquens |
1.1.1.36 | 250000 | - |
- |
Methylorubrum rhodesianum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.36 | acetoacetyl-CoA + NADH + H+ | Zoogloea ramigera | - |
L-3-hydroxybutyryl-CoA + NAD+ | - |
? | |
1.1.1.36 | acetoacetyl-CoA + NADH + H+ | Rhodospirillum rubrum | - |
L-3-hydroxybutyryl-CoA + NAD+ | - |
? | |
1.1.1.36 | acetoacetyl-CoA + NADPH + H+ | Zoogloea ramigera | PHB synthesis | D-3-hydroxybutyryl-CoA + NADP+ | - |
r | |
1.1.1.36 | acetoacetyl-CoA + NADPH + H+ | Cupriavidus necator | PHB synthesis | D-3-hydroxybutyryl-CoA + NADP+ | - |
? | |
1.1.1.36 | acetoacetyl-CoA + NADPH + H+ | Methylorubrum extorquens | tricarboxylic acid cycle, poly-3-hydroxybutyrate synthesis, aerobic serine pathway | D-3-hydroxybutyryl-CoA + NADP+ | - |
r | |
1.1.1.36 | acetoacetyl-CoA + NADPH + H+ | Methylorubrum extorquens 15 | tricarboxylic acid cycle, poly-3-hydroxybutyrate synthesis, aerobic serine pathway | D-3-hydroxybutyryl-CoA + NADP+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.36 | Azotobacter vinelandii | - |
- |
- |
1.1.1.36 | Cupriavidus necator | - |
- |
- |
1.1.1.36 | Methylorubrum extorquens | - |
15 | - |
1.1.1.36 | Methylorubrum extorquens 15 | - |
15 | - |
1.1.1.36 | Methylorubrum rhodesianum | - |
MB 126 | - |
1.1.1.36 | Methylorubrum rhodesianum MB 126 | - |
MB 126 | - |
1.1.1.36 | Rhodospirillum rubrum | - |
- |
- |
1.1.1.36 | Zoogloea ramigera | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.36 | PEG, DEAE-Toyopearl column | Methylorubrum extorquens |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.36 | 184 | - |
- |
Methylorubrum extorquens |
EC Number | Storage Stability | Organism |
---|---|---|
1.1.1.36 | 0°C, 25 mM Tris-HCl, pH 8.0 | Methylorubrum extorquens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.36 | acetoacetyl-CoA + NADH + H+ | - |
Zoogloea ramigera | L-3-hydroxybutyryl-CoA + NAD+ | - |
? | |
1.1.1.36 | acetoacetyl-CoA + NADH + H+ | - |
Cupriavidus necator | L-3-hydroxybutyryl-CoA + NAD+ | - |
? | |
1.1.1.36 | acetoacetyl-CoA + NADH + H+ | - |
Rhodospirillum rubrum | L-3-hydroxybutyryl-CoA + NAD+ | - |
? | |
1.1.1.36 | acetoacetyl-CoA + NADPH + H+ | - |
Methylorubrum rhodesianum | D-beta-hydroxybutyryl-CoA + NADP+ | - |
? | |
1.1.1.36 | acetoacetyl-CoA + NADPH + H+ | - |
Methylorubrum rhodesianum MB 126 | D-beta-hydroxybutyryl-CoA + NADP+ | - |
? | |
1.1.1.36 | acetoacetyl-CoA + NADPH + H+ | PHB synthesis | Zoogloea ramigera | D-3-hydroxybutyryl-CoA + NADP+ | - |
r | |
1.1.1.36 | acetoacetyl-CoA + NADPH + H+ | PHB synthesis | Cupriavidus necator | D-3-hydroxybutyryl-CoA + NADP+ | - |
? | |
1.1.1.36 | acetoacetyl-CoA + NADPH + H+ | tricarboxylic acid cycle, poly-3-hydroxybutyrate synthesis, aerobic serine pathway | Methylorubrum extorquens | D-3-hydroxybutyryl-CoA + NADP+ | - |
r | |
1.1.1.36 | acetoacetyl-CoA + NADPH + H+ | tricarboxylic acid cycle, poly-3-hydroxybutyrate synthesis, aerobic serine pathway | Methylorubrum extorquens 15 | D-3-hydroxybutyryl-CoA + NADP+ | - |
r | |
1.1.1.36 | acetoacetyl-CoA + NADPH + H+ | - |
Azotobacter vinelandii | (R)-3-hydroxybutanoyl-CoA +NADP+ | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.36 | tetramer | - |
Zoogloea ramigera |
1.1.1.36 | tetramer | 4 * 31000, 12% PAGE | Methylorubrum extorquens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.36 | 30 | - |
- |
Zoogloea ramigera |
1.1.1.36 | 30 | - |
- |
Methylorubrum extorquens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.36 | 7.5 | - |
- |
Methylorubrum extorquens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.36 | NADPH | - |
Azotobacter vinelandii | |
1.1.1.36 | NADPH | - |
Cupriavidus necator | |
1.1.1.36 | NADPH | - |
Methylorubrum rhodesianum | |
1.1.1.36 | NADPH | absolute specificity | Zoogloea ramigera | |
1.1.1.36 | NADPH | absolute specificity | Methylorubrum extorquens |