Literature summary extracted from

Alvarez-Villafane, E.; Soler, J.; del Valle, P.; Busto, F.; de Arriaga, D.
Two NAD+-dependent isocitrate dehydrogenase forms in Phycomyces blakesleeanus. Induction in response to acetate growth and characterization, kinetics, and regulation of both enzyme forms (1996), Biochemistry, 35, 4741-4752.

Activating Compound

EC Number	Activating Compound	Comment	Organism	Structure
1.1.1.41	AMP	activates ICDH-1	Phycomyces blakesleeanus

General Stability

EC Number	General Stability	Organism
1.1.1.41	ICDH-2 is more stable to urea than ICDH-1	Phycomyces blakesleeanus

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
1.1.1.41	NADH	inhibits ICDH-2	Phycomyces blakesleeanus

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
1.1.1.41	Mg2+	required, active substrate is Mg-DL-isocitrate chelate	Phycomyces blakesleeanus
1.1.1.41	Mn2+	requires divalent cations	Phycomyces blakesleeanus

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
1.1.1.41	40500	-	alpha8, 8 * 40500, SDS PAGE, ICDH-1 and ICDH-2	Phycomyces blakesleeanus
1.1.1.41	338000	-	gel filtration	Phycomyces blakesleeanus

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
1.1.1.41	isocitrate + NAD+	Phycomyces blakesleeanus	-	2-oxoglutarate + CO2 + NADH + H+	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.1.1.41	Phycomyces blakesleeanus	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.1.1.41	-	Phycomyces blakesleeanus

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.1.1.41	37.5	-	ICDH-1	Phycomyces blakesleeanus
1.1.1.41	48.9	-	ICDH-2	Phycomyces blakesleeanus

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.1.1.41	isocitrate + NAD+	-	Phycomyces blakesleeanus	2-oxoglutarate + CO2 + NADH + H+	-	?

Subunits

EC Number	Subunits	Comment	Organism
1.1.1.41	octamer	alpha8, 8 * 40500, SDS PAGE, ICDH-1 and ICDH-2	Phycomyces blakesleeanus

Temperature Range [°C]

EC Number	Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
1.1.1.41	30	60	-	Phycomyces blakesleeanus

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
1.1.1.41	7.5	9	-	Phycomyces blakesleeanus

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.1.1.41	NAD+	-	Phycomyces blakesleeanus

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Release 2023.1 (January 2023)