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Literature summary extracted from

  • Curry, R.A.; Ting, I.P.
    Purification, properties, and kinetic observations on the isoenzymes of NADP isocitrate dehydrogenase of maize (1976), Arch. Biochem. Biophys., 176, 501-509.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.1.42 instable in solutions of low ionic strength, stabilization by ammonium sulfate Sus scrofa
1.1.1.42 instable in solutions of low ionic strength, stabilization by ammonium sulfate Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.42 0.0017
-
D,L-isocitrate isozyme 2 Zea mays
1.1.1.42 0.003
-
NADP+ isozyme 2 Zea mays
1.1.1.42 0.0031
-
D,L-isocitrate isozyme 1 Zea mays
1.1.1.42 0.0063
-
NADP+ isozyme 1 Zea mays

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.42 mitochondrion
-
Sus scrofa 5739
-
1.1.1.42 mitochondrion
-
Geobacillus stearothermophilus 5739
-
1.1.1.42 mitochondrion
-
Zea mays 5739
-
1.1.1.42 mitochondrion
-
Azotobacter sp. 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.42 68000
-
gel filtration Sus scrofa
1.1.1.42 81000 83000 gel filtration Zea mays
1.1.1.42 105000
-
gel filtration Cereibacter sphaeroides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.42 isocitrate + NADP+ Sus scrofa
-
2-oxoglutarate + NADPH + H+ + CO2
-
?
1.1.1.42 isocitrate + NADP+ Geobacillus stearothermophilus
-
2-oxoglutarate + NADPH + H+ + CO2
-
?
1.1.1.42 isocitrate + NADP+ Cereibacter sphaeroides
-
2-oxoglutarate + NADPH + H+ + CO2
-
?
1.1.1.42 isocitrate + NADP+ Zea mays
-
2-oxoglutarate + NADPH + H+ + CO2
-
?
1.1.1.42 isocitrate + NADP+ Azotobacter sp.
-
2-oxoglutarate + NADPH + H+ + CO2
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.42 Azotobacter sp.
-
-
-
1.1.1.42 Cereibacter sphaeroides
-
i.e. Rhodobacter sphaeroides
-
1.1.1.42 Geobacillus stearothermophilus
-
-
-
1.1.1.42 Sus scrofa
-
pig
-
1.1.1.42 Zea mays
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.42
-
Zea mays

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.1.1.42 overnight dialysis against guanidine-HCl, GDP buffer Sus scrofa
1.1.1.42 overnight dialysis against guanidine-HCl, GDP buffer Geobacillus stearothermophilus
1.1.1.42 overnight dialysis against guanidine-HCl, GDP buffer Cereibacter sphaeroides
1.1.1.42 overnight dialysis against guanidine-HCl, GDP buffer Zea mays
1.1.1.42 overnight dialysis against guanidine-HCl, GDP buffer Azotobacter sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.42 heart
-
Sus scrofa
-
1.1.1.42 seed
-
Zea mays
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.42 33.5
-
-
Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.42 isocitrate + NADP+
-
Sus scrofa 2-oxoglutarate + NADPH + H+ + CO2
-
?
1.1.1.42 isocitrate + NADP+
-
Geobacillus stearothermophilus 2-oxoglutarate + NADPH + H+ + CO2
-
?
1.1.1.42 isocitrate + NADP+
-
Cereibacter sphaeroides 2-oxoglutarate + NADPH + H+ + CO2
-
?
1.1.1.42 isocitrate + NADP+
-
Zea mays 2-oxoglutarate + NADPH + H+ + CO2
-
?
1.1.1.42 isocitrate + NADP+
-
Azotobacter sp. 2-oxoglutarate + NADPH + H+ + CO2
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.42 7.4
-
-
Zea mays