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Literature summary extracted from

  • Ochiai, T.; Fukunaga, N.; Sasaki, S.
    Two structurally different NADP-specific isocitrate dehydrogenases in an obligately psychrophilic bacterium, Vibrio sp. strain ABE-1 (1984), J. Gen. Appl. Microbiol., 30, 479-487.
No PubMed abstract available

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.42 Mg2+ absolute requirement for divalent cations Vibrio sp.
1.1.1.42 Mn2+ absolute requirement for divalent cations Vibrio sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.42 85000
-
 gel filtration, isoenzyme I: dimer Vibrio sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.42 isocitrate + NADP+ Halobacterium salinarum
-
 2-oxoglutarate + NADPH + H+ + CO2
-
 ?
1.1.1.42 isocitrate + NADP+ Vibrio sp.
-
 2-oxoglutarate + NADPH + H+ + CO2
-
 ?
1.1.1.42 isocitrate + NADP+ Vibrio sp. ABE-1
-
 2-oxoglutarate + NADPH + H+ + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.42 Halobacterium salinarum
-
-
-
1.1.1.42 Vibrio sp.
-
-
-
1.1.1.42 Vibrio sp. ABE-1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.42 strain ABE-1 Vibrio sp.

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.1.1.42 with 2-mercaptoethanol after inactivation at 40°C, isoenzyme II, 40% renaturation Vibrio sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.42 isocitrate + NADP+
-
 Halobacterium salinarum 2-oxoglutarate + NADPH + H+ + CO2
-
 ?
1.1.1.42 isocitrate + NADP+
-
 Vibrio sp. 2-oxoglutarate + NADPH + H+ + CO2
-
 ?
1.1.1.42 isocitrate + NADP+
-
 Vibrio sp. ABE-1 2-oxoglutarate + NADPH + H+ + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.42 dimer isoenzyme I Vibrio sp.
1.1.1.42 monomer isoenzyme II, 1 * 85000, SDS-PAGE Vibrio sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.42 20
-
 isoenzyme II Vibrio sp.
1.1.1.42 40
-
 isoenzyme I Vibrio sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.42 15
-
 isoenzyme II Vibrio sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.42 NADP+
-
 Halobacterium salinarum
1.1.1.42 NADP+
-
 Vibrio sp.