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Literature summary extracted from

  • Muro-Pastor, M.I.; Florencio, F.J.
    Purification and properties of NADP-isocitrate dehydrogenase from the unicellular cyanobacterium Synechocystis sp. PCC 6803 (1992), Eur. J. Biochem., 203, 99-105.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.42 2-oxoglutarate non-competitive inhibitor Synechocystis sp.
1.1.1.42 5'-ADP non-competitive inhibitor Synechocystis sp.
1.1.1.42 NADPH competitive inhibitor Synechocystis sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.42 0.004
-
 Mn2+ Mn2+-dependent reaction Synechocystis sp.
1.1.1.42 0.0057
-
 D,L-isocitrate Mn2+-dependent reaction Synechocystis sp.
1.1.1.42 0.006
-
 NADP+ Mn2+-dependent raction Synechocystis sp.
1.1.1.42 0.012
-
 NADP+ Mg2+-dependent reaction Synechocystis sp.
1.1.1.42 0.059
-
 D,L-isocitrate Mg2+-dependent reaction Synechocystis sp.
1.1.1.42 0.125
-
 Mg2+ Mg2+-dependent reaction Synechocystis sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.42 Mg2+ absolute requirement for divalent cations Synechocystis sp.
1.1.1.42 Mn2+ absolute requirement for divalent cations Synechocystis sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.42 57000
-
 alpha2, 2 * 57000, SDS-PAGE Synechocystis sp.
1.1.1.42 108000
-
 gel filtration Synechocystis sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.42 isocitrate + NADP+ Synechocystis sp.
-
 2-oxoglutarate + NADPH + H+ + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.42 Synechocystis sp.
-
 PCC 6803
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.42
-
 Synechocystis sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.42 15.7
-
-
 Synechocystis sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.42 isocitrate + NADP+
-
 Synechocystis sp. 2-oxoglutarate + NADPH + H+ + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.42 dimer alpha2, 2 * 57000, SDS-PAGE Synechocystis sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.42 9
-
-
 Synechocystis sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.42 7.5 10
-
 Synechocystis sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.42 NADP+
-
 Synechocystis sp.