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Literature summary extracted from

  • Novotny, J.F.; Perry, J.J.
    Characterization of a heat-stable NADP-dependent isocitrate dehydrogenase from the obligate thermophile Thermoleophilum minutum YS-4 (1991), Appl. Microbiol. Biotechnol., 35, 461-465.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.42 p-chloromercuribenzoate
-
 Thermoleophilum minutum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.42 0.033
-
 D,L-isocitrate
-
 Thermoleophilum minutum
1.1.1.42 0.048
-
 NADP+
-
 Thermoleophilum minutum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.42 Mg2+ absolute requirement for divalent cations Thermoleophilum minutum
1.1.1.42 Mn2+ absolute requirement for divalent cations Thermoleophilum minutum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.42 30500
-
 alpha2, 2 * 30500, SDS-PAGE Thermoleophilum minutum
1.1.1.42 60000
-
 PAGE and gel filtration Thermoleophilum minutum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.42 isocitrate + NADP+ Thermoleophilum minutum
-
 2-oxoglutarate + NADPH + H+ + CO2
-
 ?
1.1.1.42 isocitrate + NADP+ Thermoleophilum minutum YS-4
-
 2-oxoglutarate + NADPH + H+ + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.42 Thermoleophilum minutum
-
 YS-4
-
1.1.1.42 Thermoleophilum minutum YS-4
-
 YS-4
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.42
-
 Thermoleophilum minutum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.42 3884
-
-
 Thermoleophilum minutum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.42 isocitrate + NADP+
-
 Thermoleophilum minutum 2-oxoglutarate + NADPH + H+ + CO2
-
 ?
1.1.1.42 isocitrate + NADP+
-
 Thermoleophilum minutum YS-4 2-oxoglutarate + NADPH + H+ + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.42 dimer alpha2, 2 * 30500, SDS-PAGE Thermoleophilum minutum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.42 75
-
-
 Thermoleophilum minutum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.42 70
-
 after 1 h incubation retains 57% of initial activity Thermoleophilum minutum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.42 7.2
-
-
 Thermoleophilum minutum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.42 NADP+
-
 Thermoleophilum minutum