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Literature summary extracted from

  • Ben-Bassat, A.; Goldberg, I.
    Purification and properties of glucose-6-phosphate dehydrogenase (NADP+/NAD+) and 6-phosphogluconate dehydrogenase (NADP+/NAD+) from methanol-grown Pseudomonas C (1980), Biochim. Biophys. Acta, 611, 1-10.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.43 ATP
-
 Enterococcus faecalis
1.1.1.43 ATP with NAD+ as coenzyme, with NADP+ as coenzyme activity is less affected Pseudomonas sp.
1.1.1.43 NADH
-
 Pseudomonas sp.
1.1.1.43 NADPH
-
 Burkholderia cepacia
1.1.1.43 NADPH
-
 Pseudomonas sp.
1.1.1.49 ATP
-
 Pseudomonas sp.
1.1.1.49 NADH
-
 Pseudomonas sp.
1.1.1.351 ATP with NAD+ as the coenzyme ATP inhibits 6-phosphogluconate dehydrogeanse activity, while with NADP+ as the coenzyme, activity is less affected Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.43 0.013
-
 NADP+
-
 [Candida] boidinii
1.1.1.43 0.02
-
 NADP+
-
 Pseudomonas sp.
1.1.1.43 0.04
-
 NAD+
-
 Pseudomonas sp.
1.1.1.43 0.1
-
 6-phospho-D-gluconate
-
 Pseudomonas sp.
1.1.1.43 0.5
-
 NAD+
-
 Pseudomonas sp.
1.1.1.49 additional information
-
 additional information
-
 Pseudomonas sp.
1.1.1.351 0.03
-
 NADP+ pH and temperature not specified in the publication Pseudomonas sp.
1.1.1.351 0.1
-
 6-phospho-D-gluconate using NADP+ as cosubstrate, pH and temperature not specified in the publication Pseudomonas sp.
1.1.1.351 0.5
-
 NAD+ pH and temperature not specified in the publication Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.43 Mg2+ 2 mM required for maximum activity Pseudomonas sp.
1.1.1.43 Mg2+ 20 mM required for maximum activity [Candida] boidinii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.43 55000
-
 4 * 55000, SDS-PAGE Pseudomonas sp.
1.1.1.43 110000
-
-
 [Candida] boidinii
1.1.1.43 220000
-
 gel filtration Pseudomonas sp.
1.1.1.49 150000
-
 gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.43 6-phospho-D-gluconate + NAD(P)+ Pseudomonas sp.
-
 6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
 r
1.1.1.43 6-phospho-D-gluconate + NAD(P)+ Enterococcus faecalis
-
 6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
 r
1.1.1.43 6-phospho-D-gluconate + NAD(P)+ [Candida] boidinii
-
 6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
 r
1.1.1.43 6-phospho-D-gluconate + NAD(P)+ Burkholderia cepacia
-
 6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
 r
1.1.1.43 6-phospho-D-gluconate + NAD(P)+ Pseudomonas sp. C
-
 6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
 r
1.1.1.43 6-phospho-D-gluconate + NAD(P)+ Pseudomonas sp. W5
-
 6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
 r
1.1.1.49 D-glucose 6-phosphate + H2O + NADP+ Pseudomonas sp.
-
 D-gluconate 6-phosphate + NADPH + H+ 6-phosphoglucono-delta-lactone r
1.1.1.49 D-glucose 6-phosphate + H2O + NADP+ Pseudomonas sp. C
-
 D-gluconate 6-phosphate + NADPH + H+ 6-phosphoglucono-delta-lactone r
1.1.1.49 D-glucose 6-phosphate + H2O + NADP+ Pseudomonas sp. W5
-
 D-gluconate 6-phosphate + NADPH + H+ 6-phosphoglucono-delta-lactone r
1.1.1.351 6-phospho-D-gluconate + NAD+ Pseudomonas sp.
-
 D-ribulose 5-phosphate + CO2 + NADH + H+
-
 ?
1.1.1.351 6-phospho-D-gluconate + NAD+ Pseudomonas sp. C
-
 D-ribulose 5-phosphate + CO2 + NADH + H+
-
 ?
1.1.1.351 6-phospho-D-gluconate + NAD+ Pseudomonas sp. W5
-
 D-ribulose 5-phosphate + CO2 + NADH + H+
-
 ?
1.1.1.351 6-phospho-D-gluconate + NADP+ Pseudomonas sp.
-
 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 ?
1.1.1.351 6-phospho-D-gluconate + NADP+ Pseudomonas sp. C
-
 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 ?
1.1.1.351 6-phospho-D-gluconate + NADP+ Pseudomonas sp. W5
-
 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.43 Burkholderia cepacia
-
 type I
-
1.1.1.43 Enterococcus faecalis
-
 type I
-
1.1.1.43 Pseudomonas sp.
-
 C
-
1.1.1.43 Pseudomonas sp.
-
 W5
-
1.1.1.43 Pseudomonas sp. C
-
 C
-
1.1.1.43 Pseudomonas sp. W5
-
 W5
-
1.1.1.43 [Candida] boidinii
-
 methanol utilizing yeast
-
1.1.1.49 Pseudomonas sp.
-
 C
-
1.1.1.49 Pseudomonas sp. C
-
 C
-
1.1.1.351 Pseudomonas sp.
-
-
-
1.1.1.351 Pseudomonas sp. C
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.43
-
 Pseudomonas sp.
1.1.1.43
-
 [Candida] boidinii
1.1.1.49
-
 Pseudomonas sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.351 culture condition:methanol-grown cell
-
 Pseudomonas sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.43 39.7
-
-
 [Candida] boidinii
1.1.1.43 56.4
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.43 6-phospho-D-gluconate + NAD(P)+
-
 Pseudomonas sp. 6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
 r
1.1.1.43 6-phospho-D-gluconate + NAD(P)+
-
 Enterococcus faecalis 6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
 r
1.1.1.43 6-phospho-D-gluconate + NAD(P)+
-
 [Candida] boidinii 6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
 r
1.1.1.43 6-phospho-D-gluconate + NAD(P)+
-
 Burkholderia cepacia 6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
 r
1.1.1.43 6-phospho-D-gluconate + NAD(P)+
-
 Pseudomonas sp. C 6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
 r
1.1.1.43 6-phospho-D-gluconate + NAD(P)+
-
 Pseudomonas sp. W5 6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
 r
1.1.1.43 D-glucose-6-phosphate + NAD+
-
 Pseudomonas sp. 2-dehydro-6-phospho-D-glucose + NADH
-
 ?
1.1.1.43 D-glucose-6-phosphate + NAD+
-
 Pseudomonas sp. C 2-dehydro-6-phospho-D-glucose + NADH
-
 ?
1.1.1.43 D-glucose-6-phosphate + NAD+
-
 Pseudomonas sp. W5 2-dehydro-6-phospho-D-glucose + NADH
-
 ?
1.1.1.49 D-glucose 6-phosphate + H2O + NADP+
-
 Pseudomonas sp. D-gluconate 6-phosphate + NADPH + H+ 6-phosphoglucono-delta-lactone r
1.1.1.49 D-glucose 6-phosphate + H2O + NADP+
-
 Pseudomonas sp. C D-gluconate 6-phosphate + NADPH + H+ 6-phosphoglucono-delta-lactone r
1.1.1.49 D-glucose 6-phosphate + H2O + NADP+
-
 Pseudomonas sp. W5 D-gluconate 6-phosphate + NADPH + H+ 6-phosphoglucono-delta-lactone r
1.1.1.351 6-phospho-D-gluconate + NAD+
-
 Pseudomonas sp. D-ribulose 5-phosphate + CO2 + NADH + H+
-
 ?
1.1.1.351 6-phospho-D-gluconate + NAD+
-
 Pseudomonas sp. C D-ribulose 5-phosphate + CO2 + NADH + H+
-
 ?
1.1.1.351 6-phospho-D-gluconate + NAD+
-
 Pseudomonas sp. W5 D-ribulose 5-phosphate + CO2 + NADH + H+
-
 ?
1.1.1.351 6-phospho-D-gluconate + NADP+
-
 Pseudomonas sp. D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 ?
1.1.1.351 6-phospho-D-gluconate + NADP+
-
 Pseudomonas sp. C D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 ?
1.1.1.351 6-phospho-D-gluconate + NADP+
-
 Pseudomonas sp. W5 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.43 tetramer 4 * 55000, SDS-PAGE Pseudomonas sp.

Synonyms

EC Number Synonyms Comment Organism
1.1.1.351 6-phospho-D-gluconate:NADP+ 2-oxidoreductase
-
 Pseudomonas sp.
1.1.1.351 6-phosphogluconate dehydrogenase (NADP+/NAD+)
-
 Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.49 30
-
 assay at Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.43 8 8.5
-
 [Candida] boidinii
1.1.1.43 8 9
-
 Pseudomonas sp.
1.1.1.49 8.5 9.5
-
 Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.43 NAD+
-
 Burkholderia cepacia
1.1.1.43 NAD+ dual specificity for both NAD+ and NADP+ Pseudomonas sp.
1.1.1.43 NADP+
-
 Burkholderia cepacia
1.1.1.43 NADP+ dual specificity for both NAD+ and NADP+ Pseudomonas sp.
1.1.1.49 NAD+
-
 Pseudomonas sp.
1.1.1.49 NADP+
-
 Pseudomonas sp.
1.1.1.49 NADPH
-
 Pseudomonas sp.
1.1.1.351 NAD+ the enzyme has a dual specificity for both NADP+ or NAD+ Pseudomonas sp.
1.1.1.351 NADP+ the enzyme has a dual specificity for both NADP+ or NAD+ Pseudomonas sp.