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Literature summary extracted from

  • Scott, W.A.; Abramsky, T.
    6-Phosphogluconate dehydrogenase from Neurospora crassa (1975), Methods Enzymol., 41, 227-231.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.44 iodoacetamide
-
 Neurospora crassa
1.1.1.44 NADPH
-
 Neurospora crassa
1.1.1.44 phosphate
-
 Neurospora crassa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.44 0.01
-
 6-phosphogluconate
-
 Neurospora crassa
1.1.1.44 0.03
-
 NADP+
-
 Neurospora crassa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.44 57000
-
 2 * 57000, SDS-PAGE Neurospora crassa
1.1.1.44 110000 120000 gel filtration Neurospora crassa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.44 6-phospho-D-gluconate + NADP+ Saccharomyces cerevisiae
-
 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
1.1.1.44 6-phospho-D-gluconate + NADP+ Neurospora crassa
-
 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.44 Neurospora crassa
-
-
-
1.1.1.44 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.44
-
 Neurospora crassa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.44 mycelium
-
 Neurospora crassa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.44 30.8
-
-
 Neurospora crassa

Storage Stability

EC Number Storage Stability Organism
1.1.1.44 5°C, purified enzyme is stable up to 3 months if care is taken to exclude molecular oxygen from the enzyme solution Neurospora crassa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.44 6-phospho-D-gluconate + NADP+
-
 Saccharomyces cerevisiae D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
1.1.1.44 6-phospho-D-gluconate + NADP+
-
 Neurospora crassa D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
1.1.1.44 additional information neither gluconic acid nor glucose-6-phosphate can replace 6-phosphogluconate as substrate Neurospora crassa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.44 dimer 2 * 57000, SDS-PAGE Neurospora crassa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.44 7.8 9
-
 Neurospora crassa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.44 NADP+ NAD+ is completely ineffective as hydrogen acceptor Neurospora crassa