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Literature summary extracted from

  • Cannistraro, V.J.; Borack, L.I.; Chase, T.
    Subunit structure and kinetic properties of L-beta-hydroxyacid dehydrogenase of Drosophila (1979), Biochim. Biophys. Acta, 569, 1-5.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.1.45 3 M urea results in loss of activity of 83% in 7 min Drosophila melanogaster

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.45 acetoacetate competitive inhibitor Drosophila melanogaster
1.1.1.45 NADH competitive inhibitor Drosophila melanogaster

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.45 0.09
-
 NAD+ cosubstrate L-gulonate, pH 8.2 Drosophila melanogaster
1.1.1.45 0.13
-
 NAD+ cosubstrate L-gulonate, pH 8.2 Drosophila melanogaster
1.1.1.45 0.85
-
 L-gulonate cofactor NAD+, pH 8.2 Drosophila melanogaster
1.1.1.45 14.9
-
 L-3-hydroxybutanoate cofactor NAD+, pH 8.2 Drosophila melanogaster

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.45 23500
-
 alphabeta, 1 * 23500 + 1 * 40000, SDS-PAGE Drosophila melanogaster
1.1.1.45 40000
-
 alphabeta, 1 * 23500 + 1 * 40000, SDS-PAGE Drosophila melanogaster
1.1.1.45 63500
-
 SDS-PAGE Drosophila melanogaster

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.45 L-gulonic acid + NAD+ Drosophila melanogaster
-
 3-dehydro-L-gulonic acid + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.45 Drosophila melanogaster
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.45 12
-
-
 Drosophila melanogaster

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.45 DL-3-hydroxybutanoate + NADH + H+
-
 Drosophila melanogaster acetoacetate + NAD+
-
 r
1.1.1.45 L-3-hydroxybutanoate + NADH
-
 Drosophila melanogaster acetoacetate + NAD+
-
 ?
1.1.1.45 L-gulonic acid + NAD+
-
 Drosophila melanogaster 3-dehydro-L-gulonic acid + NADH + H+
-
 ?
1.1.1.45 L-gulonic acid + NAD+
-
 Drosophila melanogaster 3-dehydro-L-gulonic acid + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.45 dimer alphabeta, 1 * 23500 + 1 * 40000, SDS-PAGE Drosophila melanogaster

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.45 0.733
-
 DL-3-hydroxybutanoate
-
 Drosophila melanogaster
1.1.1.45 7.4
-
 L-gulonate
-
 Drosophila melanogaster

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.45 8.2
-
-
 Drosophila melanogaster

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.45 NAD+
-
 Drosophila melanogaster
1.1.1.45 NADH
-
 Drosophila melanogaster
1.1.1.45 NADP+ 10% activity of NAD+ Drosophila melanogaster