EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.54 | sequence homology with other NADPH-dependent oxidoreductases from plants | Nicotiana tabacum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.1.102 | additional information | - |
additional information | the Km-values for the (S)-alcohols are one order of magnitude lower than those for the (R)-alcohols, indicating that the enzyme has a higher affinity for the S-configuration than for the R-configuration | Nicotiana tabacum | |
1.3.1.102 | 1.9 | - |
(2S,4R)-carveol | pH 8.0, 35°C, NADP+ | Nicotiana tabacum | |
1.3.1.102 | 2.5 | - |
(2S,4S)-carveol | pH 8.0, 35°C, NADP+ | Nicotiana tabacum | |
1.3.1.102 | 38 | - |
(2R,4R)-carveol | pH 8.0, 35°C, NADP+ | Nicotiana tabacum | |
1.3.1.102 | 52 | - |
(2R,4S)-carveol | pH 8.0, 35°C, NADP+ | Nicotiana tabacum | |
1.3.1.102 | 590 | - |
(1R,2S,4S)-neoisodihydrocarveol | pH 8.0, 35°C, NADP+ | Nicotiana tabacum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.54 | 37000 | - |
2 * 37000, SDS-PAGE and amino acid sequence analysis | Nicotiana tabacum |
1.1.1.54 | 74000 | - |
gel filtration | Nicotiana tabacum |
1.3.1.102 | 37000 | - |
alpha2, 2 * 37000, determined by SDS-PAGE | Nicotiana tabacum |
1.3.1.102 | 38080 | - |
allyl-ADH1, calculated from sequence | Nicotiana tabacum |
1.3.1.102 | 74000 | - |
approximately, gel filtration chromatography on a TSK G3000SW HPLC column | Nicotiana tabacum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.54 | allyl alcohol + NADP+ | Nicotiana tabacum | - |
acrolein + NADPH | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.54 | Nicotiana tabacum | - |
- |
- |
1.3.1.102 | Nicotiana tabacum | Q9SLN8 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.54 | - |
Nicotiana tabacum |
1.3.1.102 | by DEAE-Toyopearl anion exchange column chromatography, followed by Red-Toyopearl affinity column chromatography | Nicotiana tabacum |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.54 | 0.11 | - |
- |
Nicotiana tabacum |
1.3.1.102 | 0.11 | - |
Red-Toyopearl affinity column chromatography, pH 8.0, 35°C | Nicotiana tabacum |
1.3.1.102 | 4.6 | - |
DEAE-Toyopearl anion exchange column chromatography, pH 8.0, 35°C | Nicotiana tabacum |
1.3.1.102 | 5300 | - |
crude extract, pH 8.0, 35°C | Nicotiana tabacum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.54 | (2S,4S)-carveol + NADP+ | - |
Nicotiana tabacum | (S)-carvone + NADPH | - |
r | |
1.1.1.54 | (S)-carvone + NADPH | - |
Nicotiana tabacum | (2S,4S)-carveol + NADP+ | - |
r | |
1.1.1.54 | allyl alcohol + NADP+ | - |
Nicotiana tabacum | acrolein + NADPH | - |
r | |
1.1.1.54 | allyl alcohol + NADP+ | specific for the (S)-isomer of secondary allylic alcohols, several stereomers tested for activity in both reaction directions | Nicotiana tabacum | acrolein + NADPH | - |
r | |
1.3.1.102 | (1R,2S,4S)-neoisodihydrocarveol + NADP+ | - |
Nicotiana tabacum | (1R,4S)-isodihydrocarvone + NADPH + H+ | - |
r | |
1.3.1.102 | (2R,4R)-carveol + NADP+ | - |
Nicotiana tabacum | (R)-carvone + NADPH + H+ | - |
r | |
1.3.1.102 | (2R,4S)-carveol + NADP+ | - |
Nicotiana tabacum | (S)-carvone + NADPH + H+ | - |
r | |
1.3.1.102 | (2S,4R)-carveol + NADP+ | - |
Nicotiana tabacum | (R)-carvone + NADPH + H+ | - |
r | |
1.3.1.102 | (2S,4S)-carveol + NADP+ | - |
Nicotiana tabacum | (S)-carvone + NADPH + H+ | - |
r | |
1.3.1.102 | additional information | results indicate that the enzyme catalyzes specifically the dehydrogenation of the secondary alcohols adjacent to the C-C double bond, no oxidation of saturated alcohols | Nicotiana tabacum | ? | - |
? | |
1.3.1.102 | additional information | the enzymatic reaction is a reversible oxidoreduction and the reaction is enantioselective | Nicotiana tabacum | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.54 | dimer | 2 * 37000, SDS-PAGE and amino acid sequence analysis | Nicotiana tabacum |
1.3.1.102 | homodimer | alpha2, 2 * 37000, determined by SDS-PAGE | Nicotiana tabacum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.1.102 | allyl-ADH | - |
Nicotiana tabacum |
1.3.1.102 | allyl-ADH1 | the amino acid sequences of the deduced allyl-ADHs are different in four amino acid residues | Nicotiana tabacum |
1.3.1.102 | allyl-ADH2 | the amino acid sequences of the deduced allyl-ADHs are different in four amino acid residues, allyl-ADH2 has the following mutations compared to ADH1: T17A, V30A, L345P,R278H | Nicotiana tabacum |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.1.102 | 0.003 | - |
(1R,2S,4S)-neoisodihydrocarveol | dehydrogenation, pH 8.0, 35°C, NADP+ | Nicotiana tabacum | |
1.3.1.102 | 0.003 | - |
(1R,4S)-isodihydrocarvone | hydrogenation to (1R,2S,4S)-neoisodihydrocarveol, pH 7.4, 35°C, NADP+ | Nicotiana tabacum | |
1.3.1.102 | 0.01 | - |
(S)-carvone | hydrogenation to (2R,4S)-carveol, pH 7.4, 35°C, NADP+ | Nicotiana tabacum | |
1.3.1.102 | 0.012 | - |
(R)-carvone | hydrogenation to (2R,4R)-carveol, pH 7.4, 35°C, NADP+ | Nicotiana tabacum | |
1.3.1.102 | 0.02 | - |
(2R,4R)-carveol | dehydrogenation, pH 8.0, 35°C, NADP+ | Nicotiana tabacum | |
1.3.1.102 | 0.025 | - |
(2R,4S)-carveol | dehydrogenation, pH 8.0, 35°C, NADP+ | Nicotiana tabacum | |
1.3.1.102 | 0.025 | - |
(S)-carvone | hydrogenation to (2S,4S)-carveol, pH 7.4, 35°C, NADP+ | Nicotiana tabacum | |
1.3.1.102 | 0.032 | - |
(R)-carvone | hydrogenation to (2S,4R)-carveol, pH 7.4, 35°C, NADP+ | Nicotiana tabacum | |
1.3.1.102 | 0.077 | - |
(2S,4S)-carveol | dehydrogenation, pH 8.0, 35°C, NADP+ | Nicotiana tabacum | |
1.3.1.102 | 0.097 | - |
(2S,4R)-carveol | dehydrogenation, pH 8.0, 35°C, NADP+ | Nicotiana tabacum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.54 | 7.4 | - |
reduction of carbonyl group of (S)-carvone | Nicotiana tabacum |
1.1.1.54 | 8 | - |
dehydrogenation of (S)-carveol | Nicotiana tabacum |
1.3.1.102 | 7.4 | - |
reduction of the carbonyl group of (S)-carvone | Nicotiana tabacum |
1.3.1.102 | 8 | - |
dehydrogenation of (2S,4S)-carveol | Nicotiana tabacum |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.54 | NAD(P)H | - |
Nicotiana tabacum | |
1.1.1.54 | NADP+ | activity 4times greater than with NAD+ | Nicotiana tabacum | |
1.3.1.102 | NAD+ | utilization of NADP+ by the enzyme is four times greater than for NAD+ | Nicotiana tabacum | |
1.3.1.102 | NADP+ | utilization of NADP+ by the enzyme is four times greater than for NAD+ | Nicotiana tabacum |