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Literature summary extracted from

  • Hickman, J.; Ashwell, G.
    Uronic acid metabolism in bacteria. II Purification and properties of D-altronic acid and D-mannonic acid dehydrogenase in Escherichia coli (1960), J. Biol. Chem., 235, 1566-1570.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.57 2-mercaptoethanol
-
 Escherichia coli
1.1.1.57 EDTA
-
 Escherichia coli

General Stability

EC Number General Stability Organism
1.1.1.57 rapid loss of activity upon dialysis and mild heating Escherichia coli
1.1.1.57 stabilized by 1 mM cysteine at pH 7 Escherichia coli
1.1.1.57 unstable on freezing and thawing Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.57 p-chloromercuribenzoate complete inhibition at 0.03 mM Escherichia coli
1.1.1.58 p-chloromercuribenzoate slight inhibition, no inhibition below 10 mM Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.57 1
-
 D-fructuronate
-
 Escherichia coli
1.1.1.58 0.1
-
 D-tagaturonate
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.57 D-mannonate + NAD+ Escherichia coli
-
 D-fructuronate + NADH
-
 r
1.1.1.58 D-altronate + NAD+ Escherichia coli
-
 D-tagaturonate + NADH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.57 Escherichia coli
-
-
-
1.1.1.58 Escherichia coli
-
 ATCC 9637
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.57
-
 Escherichia coli
1.1.1.58
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.57 26
-
 crude extract Escherichia coli
1.1.1.57 770
-
 purified enzyme Escherichia coli
1.1.1.58 68
-
 crude extract Escherichia coli
1.1.1.58 2730
-
 purified enzyme Escherichia coli

Storage Stability

EC Number Storage Stability Organism
1.1.1.57 3°C, pH 7, 1 mM cysteine, stable for 1-2 weeks Escherichia coli
1.1.1.58 3°C or frozen at -10°C, 1 month Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.57 D-altronate + NAD+ approximately equal activity towards both fructuronate and tagaturonate in the forward reaction, and towards D-mannonate and D-altronate in the reverse reaction Escherichia coli D-tagaturonate + NADH
-
 r
1.1.1.57 D-mannonate + NAD+
-
 Escherichia coli D-fructuronate + NADH
-
 r
1.1.1.58 D-altronate + NAD+
-
 Escherichia coli D-tagaturonate + NADH
-
 r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.57 6
-
-
 Escherichia coli
1.1.1.58 6
-
-
 Escherichia coli

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.57 6.5
-
 unstable at lower pH Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.57 NAD+
-
 Escherichia coli
1.1.1.57 NADH
-
 Escherichia coli
1.1.1.58 NAD+
-
 Escherichia coli
1.1.1.58 NADH
-
 Escherichia coli