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Literature summary extracted from

  • Endahl, G.L.; Kochakian, C.D.; Hamm, D.
    Separation of a triphosphopyridine nucleotide-specific from a diphosphopyridine nucleotide-specific 17beta-hydroxy-(testosterone)dehydrogenase of guinea pig liver (1960), J. Biol. Chem., 235, 2793-2796.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.239 Ag+ 0.1 mM, complete inhibition Cavia porcellus
1.1.1.239 CuCl2 1 mM, complete inhibition Cavia porcellus
1.1.1.239 FeCl3 10 mM, 46% inhibition Cavia porcellus
1.1.1.239 Hg2+ 0.1 mM, complete inhibition Cavia porcellus
1.1.1.239 NaCN 1 mM, 50% inhibition Cavia porcellus
1.1.1.239 p-chloromercuribenzoate 0.1 mM, complete inhibition Cavia porcellus
1.1.1.239 Sodium amytal 10 mM, 25% inhibition Cavia porcellus
1.1.1.239 ZnCl2 10 mM, 10% inhibition Cavia porcellus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.239 0.008
-
 testosterone
-
 Cavia porcellus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.239 testosterone + NAD+ Cavia porcellus
-
 androst-4-ene-3,17-dione + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.239 Cavia porcellus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.239 liver
-
 Cavia porcellus
-

Storage Stability

EC Number Storage Stability Organism
1.1.1.239 4°C-6°C, 60 mM sodium diphosphate, pH 9.5, a few days, no loss in activity Cavia porcellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.239 testosterone + NAD+
-
 Cavia porcellus androst-4-ene-3,17-dione + NADH + H+
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.239 9.6
-
 sharp drop above Cavia porcellus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.239 8 9.6
-
 Cavia porcellus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.239 NAD+
-
 Cavia porcellus