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Literature summary extracted from

  • Endahl, G.L.; Kochakian, C.D.; Hamm, D.
    Separation of a triphosphopyridine nucleotide-specific from a diphosphopyridine nucleotide-specific 17beta-hydroxy-(testosterone)dehydrogenase of guinea-pig liver (1960), J. Biol. Chem., 235, 2792-2796.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.64 CuCl2 10 mM, 40% inhibition Cavia porcellus
1.1.1.64 FeCl3 10 mM, 54% inhibition Cavia porcellus
1.1.1.64 p-chloromercuribenzoate 10 mM, strong inhibition, 5% residual activity is reversed to 65% activity by either 1 mM glutathione or cysteine Cavia porcellus
1.1.1.64 Pb(NO3)2 10 mM, 30% inhibition Cavia porcellus
1.1.1.64 Sodium amytal 10 mM, 25% inhibition Cavia porcellus
1.1.1.64 Sodium cyanide 10 mM, progressive and marked inhibition Cavia porcellus
1.1.1.64 ZnCl2 10 mM, 90% inhibition Cavia porcellus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.64 0.0596
-
 testosterone enzyme activity in microsomal fraction after centrifugation Cavia porcellus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.64 microsome
-
 Cavia porcellus
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.64 17beta-estradiol + NADP+ Cavia porcellus
-
 estrone + NADPH
-
 r
1.1.1.64 testosterone + NADP+ Cavia porcellus
-
 androst-4-ene-3,17-dione + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.64 Cavia porcellus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.64 liver
-
 Cavia porcellus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.64 96.5
-
 enzyme activity in microsomal fraction after centrifugation Cavia porcellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.64 17beta-estradiol + NADP+
-
 Cavia porcellus estrone + NADPH
-
 r
1.1.1.64 testosterone + NADP+
-
 Cavia porcellus androst-4-ene-3,17-dione + NADPH + H+
-
 r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.64 10
-
 sharp drop of activity above Cavia porcellus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.64 8.5 10.5
-
 Cavia porcellus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.64 NADP+
-
 Cavia porcellus