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Literature summary extracted from

  • Schneider, K.H.; Giffhorn, F.
    Purification and properties of a polyol dehydrogenase from the phototrophic bacterium Rhodobacter sphaeroides (1989), Eur. J. Biochem., 184, 15-19.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.67 0.01 0.079 NADH
-
 Cereibacter sphaeroides
1.1.1.67 0.18 0.27 NAD+
-
 Cereibacter sphaeroides
1.1.1.67 0.29 21.8 D-mannitol
-
 Cereibacter sphaeroides
1.1.1.67 1.8 6.5 D-arabinitol
-
 Cereibacter sphaeroides
1.1.1.67 16.3 79.2 D-fructose
-
 Cereibacter sphaeroides
1.1.1.67 32
-
 D-glucitol
-
 Cereibacter sphaeroides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.67 47200
-
 sucrose density gradient centrifugation Cereibacter sphaeroides
1.1.1.67 52200
-
 1 * 52200, SDS-PAGE   Cereibacter sphaeroides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.67 D-mannitol + NAD+ Cereibacter sphaeroides
-
 D-fructose + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.67 Cereibacter sphaeroides
-
 phototrophic bacterium
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.67
-
 Cereibacter sphaeroides

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.67 60.3
-
-
 Cereibacter sphaeroides

Storage Stability

EC Number Storage Stability Organism
1.1.1.67 4°C, 1 month Cereibacter sphaeroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.67 D-arabinitol + NAD+
-
 Cereibacter sphaeroides D-ribulose + NADH + H+
-
 ?
1.1.1.67 D-glucitol + NAD+
-
 Cereibacter sphaeroides ? + NADH
-
 ?
1.1.1.67 D-mannitol + NAD+
-
 Cereibacter sphaeroides D-fructose + NADH + H+
-
 ?
1.1.1.67 D-mannitol + NAD+
-
 Cereibacter sphaeroides D-fructose + NADH + H+
-
 r
1.1.1.67 L-sorbitol + NAD+
-
 Cereibacter sphaeroides L-sorbose + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.67 monomer 1 * 52200, SDS-PAGE   Cereibacter sphaeroides

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.67 40
-
 not stable above Cereibacter sphaeroides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.67 6 7.2 D-fructose reduction Cereibacter sphaeroides
1.1.1.67 8.9 10 D-mannitol oxidation Cereibacter sphaeroides

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.67 NAD+
-
 Cereibacter sphaeroides
1.1.1.67 NADH
-
 Cereibacter sphaeroides