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Literature summary extracted from

  • Wales, M.R.; Fewson, C.A.
    NADP-dependent alcohol dehydrogenases in bacteria and yeast: purification and partial characterization of the enzymes from Acinetobacter calcoaceticus and Saccharomyces cerevisiae (1994), Microbiology, 140, 173-183.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.71 4-chloromercuribenzoate 0.01 mM, complete inhibition Acinetobacter calcoaceticus
1.1.1.71 4-chloromercuribenzoate 0.01 mM, complete inhibition; some protection of partial inhibition resulting from 0.001 mM 4-chloromercuribenzoate in the presence of 1 mM NADP+, almost complete protection with 10 mM hexan-1-ol Saccharomyces cerevisiae
1.1.1.71 EDTA 10 mM, 20% inhibition Saccharomyces cerevisiae
1.1.1.71 iodoacetamide 1 mM, complete inhibition Saccharomyces cerevisiae
1.1.1.71 N-ethylmaleimide 1 mM, complete inhibition Saccharomyces cerevisiae
1.1.1.71 Sodium azide 10 mM, 40% inhibition Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.71 0.00079
-
NADPH
-
Saccharomyces cerevisiae
1.1.1.71 0.00433
-
NADPH
-
Acinetobacter calcoaceticus
1.1.1.71 0.0202
-
NADP+
-
Saccharomyces cerevisiae
1.1.1.71 0.038
-
NADP+
-
Acinetobacter calcoaceticus
1.1.1.71 0.75
-
Butanal
-
Saccharomyces cerevisiae
1.1.1.71 1.09
-
heptan-1-ol
-
Acinetobacter calcoaceticus
1.1.1.71 1.22
-
hexan-1-ol
-
Acinetobacter calcoaceticus
1.1.1.71 3.97
-
Butanal
-
Acinetobacter calcoaceticus
1.1.1.71 5.8
-
pentan-1-ol
-
Acinetobacter calcoaceticus
1.1.1.71 6.2
-
octan-1-ol
-
Saccharomyces cerevisiae
1.1.1.71 6.8
-
hexan-1-ol
-
Saccharomyces cerevisiae
1.1.1.71 14.1
-
pentan-1-ol
-
Saccharomyces cerevisiae
1.1.1.71 52.7
-
butan-1-ol
-
Acinetobacter calcoaceticus
1.1.1.71 78.6
-
butan-1-ol
-
Saccharomyces cerevisiae
1.1.1.71 150
-
benzyl alcohol
-
Acinetobacter calcoaceticus
1.1.1.71 727
-
ethanol
-
Acinetobacter calcoaceticus
1.1.1.71 4790
-
ethanol
-
Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.71 40300
-
4 * 40300, SDS-PAGE Acinetobacter calcoaceticus
1.1.1.71 43500
-
1 * 43500, SDS-PAGE Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.71 Acinetobacter calcoaceticus
-
strains NCIB 8250 and HO1-N
-
1.1.1.71 Bacillus subtilis
-
-
-
1.1.1.71 Bacillus subtilis NCIMB 3610
-
-
-
1.1.1.71 Escherichia coli
-
-
-
1.1.1.71 Escherichia coli ML30
-
-
-
1.1.1.71 Mycobacterium tuberculosis
-
var. bosis
-
1.1.1.71 Pseudomonas aeruginosa
-
strains NCIMB 10548 and 196A
-
1.1.1.71 Pseudomonas putida
-
NCIMB 9494
-
1.1.1.71 Pseudomonas putida NCIMB 9494
-
NCIMB 9494
-
1.1.1.71 Rhodococcus rhodochrous
-
-
-
1.1.1.71 Rhodococcus rhodochrous NCIMB 13259
-
-
-
1.1.1.71 Rhodotorula graminis
-
-
-
1.1.1.71 Rhodotorula graminis KGX 39
-
-
-
1.1.1.71 Saccharomyces cerevisiae
-
D273-10B
-
1.1.1.71 Saccharomyces cerevisiae D273-10B
-
D273-10B
-
1.1.1.71 Streptomyces rimosus
-
-
-
1.1.1.71 Streptomyces rimosus 4018
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.71 DEAE-Sephacel, ammonium sulfate, Sephacryl S300HR, Phenyl-Sepharose, Red A Saccharomyces cerevisiae
1.1.1.71 DEAE-Sephacel, CM-cellulose, Sephacryl S300HR, Phenyl-Sepharose, Blue A/DEAE-Sephacel Acinetobacter calcoaceticus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.71 a primary alcohol + NAD(P)+ = an aldehyde + NAD(P)H + H+ enzyme is stereospecific for the pro-R hydride of NADPH Saccharomyces cerevisiae
1.1.1.71 a primary alcohol + NAD(P)+ = an aldehyde + NAD(P)H + H+ enzyme is stereospecific for the pro-R hydride of NADPH Acinetobacter calcoaceticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.71 allyl alcohol + NADP+ 28% of activity with hexan-1-ol Acinetobacter calcoaceticus acrylaldehyde + NADPH
-
r
1.1.1.71 benzyl alcohol + NADP+
-
Acinetobacter calcoaceticus benzaldehyde + NADPH
-
r
1.1.1.71 butan-1,4-diol + NADP+ 29% of activity with hexan-1-ol Acinetobacter calcoaceticus butandialdehyde + NADPH
-
r
1.1.1.71 butan-1-ol + NADP+ 36.3% of activity with hexan-1-ol Acinetobacter calcoaceticus butanal + NADPH
-
r
1.1.1.71 butan-1-ol + NADP+
-
Saccharomyces cerevisiae butanal + NADPH + H+
-
r
1.1.1.71 butan-1-ol + NADP+
-
Saccharomyces cerevisiae D273-10B butanal + NADPH + H+
-
r
1.1.1.71 butanal + NADPH + H+
-
Saccharomyces cerevisiae butan-1-ol + NADP+
-
r
1.1.1.71 butanal + NADPH + H+
-
Saccharomyces cerevisiae D273-10B butan-1-ol + NADP+
-
r
1.1.1.71 cis-hex-3-en-1-ol + NADP+ 18.7% of activity with hexan-1ol Acinetobacter calcoaceticus cis-hex-3-en-1-al + NADPH
-
r
1.1.1.71 decan-1-ol + NADP+ decrease in enzyme activity with increase in chain length Acinetobacter calcoaceticus decanal + NADPH
-
r
1.1.1.71 dodecan-1-ol + NADP+ decrease in enzyme activity with increase in chain length Acinetobacter calcoaceticus dodecanal + NADPH
-
r
1.1.1.71 ethanediol + NADP+ 8.5% of activity with hexan-1-ol Acinetobacter calcoaceticus glyoxal + NADPH
-
r
1.1.1.71 ethanol + NADP+
-
Saccharomyces cerevisiae acetaldehyde + NADPH + H+
-
r
1.1.1.71 ethanol + NADP+
-
Saccharomyces cerevisiae D273-10B acetaldehyde + NADPH + H+
-
r
1.1.1.71 ethanol + NADP+ 8.5% of activity with hexan-1-ol Acinetobacter calcoaceticus ethanal + NADPH + H+
-
r
1.1.1.71 heptan-1-ol + NADP+
-
Acinetobacter calcoaceticus heptanal + NADPH
-
r
1.1.1.71 hexan-1-ol + NADP+
-
Bacillus subtilis hexanal + NADPH
-
?
1.1.1.71 hexan-1-ol + NADP+
-
Escherichia coli hexanal + NADPH
-
?
1.1.1.71 hexan-1-ol + NADP+
-
Saccharomyces cerevisiae hexanal + NADPH
-
r
1.1.1.71 hexan-1-ol + NADP+
-
Rhodococcus rhodochrous hexanal + NADPH
-
?
1.1.1.71 hexan-1-ol + NADP+
-
Pseudomonas aeruginosa hexanal + NADPH
-
?
1.1.1.71 hexan-1-ol + NADP+
-
Pseudomonas putida hexanal + NADPH
-
?
1.1.1.71 hexan-1-ol + NADP+
-
Acinetobacter calcoaceticus hexanal + NADPH
-
?
1.1.1.71 hexan-1-ol + NADP+
-
Acinetobacter calcoaceticus hexanal + NADPH
-
r
1.1.1.71 hexan-1-ol + NADP+
-
Mycobacterium tuberculosis hexanal + NADPH
-
?
1.1.1.71 hexan-1-ol + NADP+
-
Rhodotorula graminis hexanal + NADPH
-
?
1.1.1.71 hexan-1-ol + NADP+
-
Streptomyces rimosus hexanal + NADPH
-
?
1.1.1.71 hexan-1-ol + NADP+
-
Streptomyces rimosus 4018 hexanal + NADPH
-
?
1.1.1.71 hexan-1-ol + NADP+
-
Rhodococcus rhodochrous NCIMB 13259 hexanal + NADPH
-
?
1.1.1.71 hexan-1-ol + NADP+
-
Pseudomonas putida NCIMB 9494 hexanal + NADPH
-
?
1.1.1.71 hexan-1-ol + NADP+
-
Rhodotorula graminis KGX 39 hexanal + NADPH
-
?
1.1.1.71 hexan-1-ol + NADP+
-
Saccharomyces cerevisiae D273-10B hexanal + NADPH
-
r
1.1.1.71 hexan-1-ol + NADP+
-
Bacillus subtilis NCIMB 3610 hexanal + NADPH
-
?
1.1.1.71 hexan-1-ol + NADP+
-
Escherichia coli ML30 hexanal + NADPH
-
?
1.1.1.71 mannitol + NADP+ 6.5% of activity with hexan-1-ol Acinetobacter calcoaceticus D-mannose + NADPH
-
r
1.1.1.71 octan-1-ol + NADP+ decrease in enzyme activity with increase in chain length Acinetobacter calcoaceticus octanal + NADPH
-
r
1.1.1.71 octan-1-ol + NADP+
-
Saccharomyces cerevisiae octanal + NADPH + H+
-
r
1.1.1.71 pentan-1-ol + NADP+
-
Saccharomyces cerevisiae pentanal + NADPH
-
?
1.1.1.71 pentan-1-ol + NADP+ 4% of activity with hexan-1-ol Acinetobacter calcoaceticus pentanal + NADPH
-
r
1.1.1.71 pentan-1-ol + NADP+
-
Saccharomyces cerevisiae D273-10B pentanal + NADPH
-
?
1.1.1.71 propan-1-ol + NADP+ 28% of activity with hexan-1-ol Acinetobacter calcoaceticus propanal + NADPH
-
r
1.1.1.71 ribitol + NADP+ 4% of activity with hexan-1-ol Acinetobacter calcoaceticus D-ribose + NADPH
-
r

Subunits

EC Number Subunits Comment Organism
1.1.1.71 monomer 1 * 43500, SDS-PAGE Saccharomyces cerevisiae
1.1.1.71 tetramer 4 * 40300, SDS-PAGE Acinetobacter calcoaceticus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.71 13.9
-
ethanol
-
Acinetobacter calcoaceticus
1.1.1.71 19.6
-
Butanal
-
Saccharomyces cerevisiae
1.1.1.71 25.4
-
octan-1-ol
-
Acinetobacter calcoaceticus
1.1.1.71 26.3
-
Butanal
-
Acinetobacter calcoaceticus
1.1.1.71 35.1
-
ethanol
-
Saccharomyces cerevisiae
1.1.1.71 38.3
-
butan-1-ol
-
Acinetobacter calcoaceticus
1.1.1.71 38.9
-
heptan-1-ol
-
Acinetobacter calcoaceticus
1.1.1.71 39.4
-
hexan-1-ol
-
Acinetobacter calcoaceticus
1.1.1.71 39.8
-
octan-1-ol
-
Saccharomyces cerevisiae
1.1.1.71 40.3
-
hexan-1-ol
-
Saccharomyces cerevisiae
1.1.1.71 44.7
-
pentan-1-ol
-
Acinetobacter calcoaceticus
1.1.1.71 50.6
-
butan-1-ol
-
Saccharomyces cerevisiae
1.1.1.71 62.9
-
pentan-1-ol
-
Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.71 6.8
-
hexanal reduction Saccharomyces cerevisiae
1.1.1.71 6.8
-
hexanal reduction Acinetobacter calcoaceticus
1.1.1.71 10.2
-
hexan-1-ol oxidation Acinetobacter calcoaceticus
1.1.1.71 10.7
-
hexan-1-ol oxidation Saccharomyces cerevisiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.71 6.4 7.15 hexanal reduction, 95% of maximal rate Saccharomyces cerevisiae
1.1.1.71 6.4 7.15 hexanal reduction, 95% of maximal rate Acinetobacter calcoaceticus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.71 NADPH
-
Bacillus subtilis
1.1.1.71 NADPH
-
Escherichia coli
1.1.1.71 NADPH
-
Saccharomyces cerevisiae
1.1.1.71 NADPH
-
Rhodococcus rhodochrous
1.1.1.71 NADPH
-
Pseudomonas aeruginosa
1.1.1.71 NADPH
-
Pseudomonas putida
1.1.1.71 NADPH
-
Acinetobacter calcoaceticus
1.1.1.71 NADPH
-
Mycobacterium tuberculosis
1.1.1.71 NADPH
-
Rhodotorula graminis
1.1.1.71 NADPH
-
Streptomyces rimosus