Literature summary extracted from

Jacquot, J.P.P.; Buchanan, B.B.; Martin, F.; Vidal, J.
Enzyme regulation in C4 photosynthesis. Purification and properties of thioredoxin-linked NADP-malate dehydrogenase from corn leaves (1981), Plant Physiol., 68, 300-304.

Activating Compound

EC Number	Activating Compound	Comment	Organism	Structure
1.1.1.82	thioredoxin	activated by thioredoxin m that is reduced either photochemically with ferredoxin and ferredoxin-thioredoxin reductase or chemically with dithiothreitol	Zea mays

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
1.1.1.82	50000	60000	unactivated enzyme, gel filtration	Zea mays

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.1.1.82	Zea mays	-	-	-

Oxidation Stability

EC Number	Oxidation Stability	Organism
1.1.1.82	the enzyme undergoes reversible oxidation/reduction during its photoregulation	Zea mays

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.1.1.82	-	Zea mays

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
1.1.1.82	leaf	-	Zea mays	-

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.1.1.82	160	-	-	Zea mays

Storage Stability

EC Number	Storage Stability	Organism
1.1.1.82	-15°C, 37.5 mM Na-acetate, pH 5.5, 0.37 mM EDTA, 25% glycerol, no significant loss of activity after 3 months	Zea mays

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.1.1.82	oxaloacetate + NADH	utilization of NADH to NADPH in reduction of oxaloacetate is 1:160	Zea mays	(S)-malate + NAD+	-	?
1.1.1.82	oxaloacetate + NADPH	-	Zea mays	(S)-malate + NADP+	-	?

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
1.1.1.82	7	8.5	activated enzyme	Zea mays

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Release 2023.1 (January 2023)