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Literature summary extracted from

  • Slaughter, J.C.; Davies, D.D.
    3-Phosphoglycerate dehydrogenase from seedlings of Pisum sativum (1975), Methods Enzymol., 41, 278-281.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.95 L-methionine maximum activation at 10 mM Pisum sativum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.95 L-serine
-
 Pisum sativum
1.1.1.95 p-hydroxymercuribenzoate
-
 Pisum sativum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.95 0.01
-
 NADH
-
 Pisum sativum
1.1.1.95 0.05
-
 3-phospho-D-glycerate
-
 Pisum sativum
1.1.1.95 0.125
-
 NAD+
-
 Pisum sativum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.95 3-phosphoglycerate + NAD+ Pisum sativum first enzyme in metabolic sequence of synthesis of serine from 3-phosphoglycerate 3-phosphohydroxypyruvate + NADH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.95 Pisum sativum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.95
-
 Pisum sativum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.95 seedling
-
 Pisum sativum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.95 11.8
-
-
 Pisum sativum

Storage Stability

EC Number Storage Stability Organism
1.1.1.95 2°C, 40% loss of activity, 7 days Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.95 3-phospho-D-glycerate + NAD+
-
 Pisum sativum 3-phosphooxypyruvate + NADH + H+
-
 r
1.1.1.95 3-phosphoglycerate + NAD+
-
 Pisum sativum 3-phosphohydroxypyruvate + NADH
-
 r
1.1.1.95 3-phosphoglycerate + NAD+ first enzyme in metabolic sequence of synthesis of serine from 3-phosphoglycerate Pisum sativum 3-phosphohydroxypyruvate + NADH
-
 r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.95 5.5
-
 NADH oxidation Pisum sativum
1.1.1.95 9.5
-
 oxidation of D-3-phosphoglycerate Pisum sativum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.95 NAD+
-
 Pisum sativum
1.1.1.95 NADH A-stereospecific for NADH Pisum sativum
1.1.1.95 NADP+ ineffective Pisum sativum
1.1.1.95 NADPH 25% of activity with NADH, can not replace NADH in standard assay concentration Pisum sativum