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Literature summary extracted from

  • Takada, Y.; Noguchi, T.; Kido, R.
    Purification, characterization and identification of aromatic 2-oxo acid reductase (1977), Life Sci., 20, 609-616.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.96 2,5-dihydroxybenzoic acid 0.5 mM, 60% inhibition Canis lupus familiaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.96 0.014
-
 NADH
-
 Canis lupus familiaris
1.1.1.96 0.04
-
 3,5-diiodo-4-hydroxyphenylpyruvate
-
 Canis lupus familiaris
1.1.1.96 0.14
-
 oxaloacetate
-
 Canis lupus familiaris
1.1.1.96 0.2
-
 Indolepyruvate
-
 Canis lupus familiaris
1.1.1.96 1.6
-
 phenylpyruvate
-
 Canis lupus familiaris

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.96 cytosol
-
 Canis lupus familiaris 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.96 40000
-
 2 * 40000, SDS-PAGE Canis lupus familiaris
1.1.1.96 75000
-
 sucrose-density gradient centrifugation Canis lupus familiaris

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.96 Canis lupus familiaris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.96 DEAE-cellulose, hydroxyapatite, isoelectric focusing, Sephadex G-150 Canis lupus familiaris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.96 brain
-
 Canis lupus familiaris
-
1.1.1.96 heart
-
 Canis lupus familiaris
-
1.1.1.96 kidney
-
 Canis lupus familiaris
-
1.1.1.96 liver
-
 Canis lupus familiaris
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.96 1200
-
-
 Canis lupus familiaris

Storage Stability

EC Number Storage Stability Organism
1.1.1.96 -20°C, 50 mM potassium phosphate, pH 7.5, 2 months, no loss of activity Canis lupus familiaris
1.1.1.96 4°C, 50 mM potassium phosphate, pH 7.5, 2 weeks, no loss of activity Canis lupus familiaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.96 3-(3,5-diiodo-4-hydroxyphenyl)pyruvate + NADH
-
 Canis lupus familiaris 3-(3,5-diiodo-4-hydroxyphenyl)lactate + NAD+
-
 r
1.1.1.96 indolepyruvate + NADH
-
 Canis lupus familiaris indolelactate + NAD+
-
 r
1.1.1.96 oxaloacetate + NADH order of activity, oxaloacetate, 3-(3,5-diiodo-4-hydroxyphenyl)pyruvate, indolepyruvate, phenylpyruvate Canis lupus familiaris malate + NAD+
-
 r
1.1.1.96 phenylpyruvate + NADH
-
 Canis lupus familiaris phenyllactate + NAD+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.96 dimer 2 * 40000, SDS-PAGE Canis lupus familiaris

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.96 NADH
-
 Canis lupus familiaris