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Literature summary extracted from

  • Phizackerley, P.J.R.
    Malate dehydrogenase (FAD-linked) from Pseudomonas ovalis Chester (1969), Methods Enzymol., 13, 135-140.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.5.4 Phospholipid activates Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.5.4 Sodium amytal 1 mM, competitive with respect to phosphatidylethanolamine, noncompetitive with respect to FAD Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.5.4 0.0024
-
 ubiquinone-9
-
 Pseudomonas putida
1.1.5.4 0.45
-
 (S)-malate
-
 Pseudomonas putida
1.1.5.4 3
-
 vitamin K3
-
 Pseudomonas putida

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.5.4 membrane firmly bound to Pseudomonas putida 16020
-

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.4 Pseudomonas putida
-
 Chester
-
1.1.5.4 Pseudomonas putida Chester
-
 Chester
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.5.4
-
 Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.5.4 9.3
-
-
 Pseudomonas putida

Storage Stability

EC Number Storage Stability Organism
1.1.5.4 -15°C, 24 h, 70% loss of activity Pseudomonas putida
1.1.5.4 0°C, 24 h, 50% loss of activity Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.4 (S)-malate + oxidized 2,6-dichlorophenol indophenol
-
 Pseudomonas putida oxaloacetate + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.5.4 (S)-malate + oxidized 2,6-dichlorophenol indophenol
-
 Pseudomonas putida Chester oxaloacetate + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.5.4 (S)-malate + ubiquinone-0
-
 Pseudomonas putida oxaloacetate + ubiquinol-0
-
 ?
1.1.5.4 (S)-malate + ubiquinone-0
-
 Pseudomonas putida Chester oxaloacetate + ubiquinol-0
-
 ?
1.1.5.4 (S)-malate + ubiquinone-6
-
 Pseudomonas putida oxaloacetate + ubiquinol-6
-
 ?
1.1.5.4 (S)-malate + ubiquinone-6
-
 Pseudomonas putida Chester oxaloacetate + ubiquinol-6
-
 ?
1.1.5.4 (S)-malate + ubiquinone-9
-
 Pseudomonas putida oxaloacetate + ubiquinol-9
-
 ?
1.1.5.4 (S)-malate + ubiquinone-9
-
 Pseudomonas putida Chester oxaloacetate + ubiquinol-9
-
 ?
1.1.5.4 (S)-malate + vitamin K1
-
 Pseudomonas putida oxaloacetate + reduced vitamin K1
-
 ?
1.1.5.4 (S)-malate + vitamin K1
-
 Pseudomonas putida Chester oxaloacetate + reduced vitamin K1
-
 ?
1.1.5.4 (S)-malate + vitamin K3
-
 Pseudomonas putida oxaloacetate + reduced vitamin K3
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.5.4 20
-
 24 h, 85% loss of activity Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.5.4 FAD Km: 0.0004 mM Pseudomonas putida