Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Imai, K.; Brodie, A.F.
    A phospholipid-requiring enzyme, malate-vitamin K reductase (1973), J. Biol. Chem., 248, 7487-7494.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.5.4 Phospholipid activity of purified enzyme is dependent on added phospholipid Mycolicibacterium phlei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.5.4 51000
-
 1 * 51000, at high salt concentrations the enzyme exists as a monomeric form which is more active than the aggregated form, SDS-PAGE Mycolicibacterium phlei
1.1.5.4 53000
-
 monomeric enzyme form, gel filtration Mycolicibacterium phlei
1.1.5.4 164000
-
 aggregated form, gel filtration Mycolicibacterium phlei

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.4 Mycolicibacterium phlei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.5.4
-
 Mycolicibacterium phlei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.5.4 52
-
-
 Mycolicibacterium phlei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.4 (S)-malate + vitamin K1
-
 Mycolicibacterium phlei oxaloacetate + reduced vitamin K1
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.5.4 monomer 1 * 51000, at high salt concentrations the enzyme exists as a monomeric form which is more active than the aggregated form, SDS-PAGE Mycolicibacterium phlei

Synonyms

EC Number Synonyms Comment Organism
1.1.5.4 malate-vitamin K reductase
-
 Mycolicibacterium phlei

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.5.4 FAD the enzyme requires FAD and vitamin K for activity Mycolicibacterium phlei
1.1.5.4 additional information no spectral evidence for the presence of a flavin or quinone in the purified enzyme Mycolicibacterium phlei
1.1.5.4 vitamin K1 the enzyme requires FAD and vitamin K for activity Mycolicibacterium phlei