Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Wang, T.P.; Lampen, J.O.
    Uracil oxidase and the isolation of barbituric acid from uracil oxidation (1952), J. Biol. Chem., 194, 785-791.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.17.99.4 methylene blue required as electron acceptor Aerobic soil bacterium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.17.99.4 cytoplasm
-
 Aerobic soil bacterium 5737
-

Organism

EC Number Organism UniProt Comment Textmining
1.17.99.4 Aerobic soil bacterium
-
-
-
1.17.99.4 Aerobic soil bacterium U-1
-
-
-

Storage Stability

EC Number Storage Stability Organism
1.17.99.4 -20°C, 80% loss of activity in crude extracts within two months Aerobic soil bacterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.99.4 5-aminouracil + acceptor
-
 Aerobic soil bacterium ?
-
 ?
1.17.99.4 5-aminouracil + acceptor
-
 Aerobic soil bacterium U-1 ?
-
 ?
1.17.99.4 thymine + acceptor
-
 Aerobic soil bacterium 5-methylbarbiturate + reduced acceptor
-
 ?
1.17.99.4 thymine + acceptor
-
 Aerobic soil bacterium U-1 5-methylbarbiturate + reduced acceptor
-
 ?
1.17.99.4 uracil + acceptor barbiturate, isobarbiturate, 5-methylbarbiturate, 6-methyluracil, dihydrothymine, dihydrouracil, 2-thiouracil, 2-thiothymine, 2-thio-5-methyluracil and cytosine are no substrates Aerobic soil bacterium barbiturate + reduced acceptor
-
 ?
1.17.99.4 uracil + acceptor acceptor: 2,6-dichlorophenolindophenol or methylene blue act as artificial electron acceptors, O2 only in the presence of methylene blue Aerobic soil bacterium barbiturate + reduced acceptor
-
 ?
1.17.99.4 uracil + acceptor barbiturate, isobarbiturate, 5-methylbarbiturate, 6-methyluracil, dihydrothymine, dihydrouracil, 2-thiouracil, 2-thiothymine, 2-thio-5-methyluracil and cytosine are no substrates Aerobic soil bacterium U-1 barbiturate + reduced acceptor
-
 ?
1.17.99.4 uracil + acceptor acceptor: 2,6-dichlorophenolindophenol or methylene blue act as artificial electron acceptors, O2 only in the presence of methylene blue Aerobic soil bacterium U-1 barbiturate + reduced acceptor
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.17.99.4 30
-
 assay at Aerobic soil bacterium

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.99.4 additional information no oxidation in the presence of O2 without methylene blue as electron acceptor Aerobic soil bacterium