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Literature summary extracted from

  • Trautwein, T.; Krauss, F.; Lottspeich, F.; Simon, H.
    The (2R)-hydroxycarboxylate-viologen-oxidoreductase from Proteus vulgaris is a molybdenum-containing iron-sulphur protein (1994), Eur. J. Biochem., 222, 1025-1032.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.99.6 KCN 5 mM, 50% inhibition after 7 min, complete inhibition after 1 h Proteus vulgaris
1.1.99.30 CN-
-
 Proteus vulgaris
1.1.99.30 O2 oxygen-sensitive Proteus vulgaris

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.99.30 membrane bound Proteus vulgaris 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.99.6 Molybdenum molybdenum containing enzyme Proteus vulgaris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.99.6 80000
-
 x * 80000, probably octamer, SDS-PAGE Proteus vulgaris
1.1.99.6 180000 600000 gel filtration, native PAGE Proteus vulgaris
1.1.99.30 80000
-
 8 * 80000, SDS-PAGE Proteus vulgaris
1.1.99.30 600000
-
 gel filtration Proteus vulgaris

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.6 Proteus vulgaris
-
-
-
1.1.99.30 Proteus vulgaris
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.1.99.6 oxygen-sensitive enzyme, has to be handled in the presence of detergent and absence of oxygen, if stirred under air at room temperatures 50% activity are lost within 30 min, the residual activity has a half-life of 10 h Proteus vulgaris

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.6 DEAE-cellulose, hydroxyapatite, gel filtration Proteus vulgaris
1.1.99.30 anaerobic conditions Proteus vulgaris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.99.30 additional information anaerobic growth conditions Proteus vulgaris
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.99.6 270
-
 80000 Da form of the enzyme Proteus vulgaris
1.1.99.6 1800
-
 600000 Da form of the enzyme Proteus vulgaris
1.1.99.30 1800
-
 purified holoenzyme Proteus vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.6 (R)-lactate + acceptor
-
 Proteus vulgaris pyruvate + reduced acceptor
-
 r
1.1.99.6 D-gluconate + acceptor
-
 Proteus vulgaris 2-oxo-gluconate + reduced acceptor
-
 r
1.1.99.6 D-glucose 6-phosphate + acceptor
-
 Proteus vulgaris ?
-
 r
1.1.99.6 N-acetylneuraminate + acceptor
-
 Proteus vulgaris ?
-
 r
1.1.99.6 phenylpyruvate + reduced acceptor
-
 Proteus vulgaris phenyl-(R)-lactate + acceptor
-
 r
1.1.99.30 a (2R)-hydroxy-carboxylate + acceptor
-
 Proteus vulgaris a 2-oxo-carboxylate + reduced acceptor
-
 ?
1.1.99.30 D-gluconate + oxidized 1,1'-carbamoyl methyl viologen anaerobic conditions Proteus vulgaris ? + reduced 1,1'-carbamoyl methyl viologen
-
 r
1.1.99.30 D-glucose 6-phosphate + oxidized 1,1'-carbamoyl methyl viologen anaerobic conditions Proteus vulgaris ? + reduced 1,1'-carbamoyl methyl viologen
-
 r
1.1.99.30 D-lactate + oxidized 1,1'-carbamoyl methyl viologen anaerobic conditions Proteus vulgaris pyruvate + reduced 1,1'-carbamoyl methyl viologen
-
 r
1.1.99.30 N-acetylneuraminate + oxidized 1,1'-carbamoyl methyl viologen anaerobic conditions Proteus vulgaris ? + reduced 1,1'-carbamoyl methyl viologen
-
 r
1.1.99.30 phenylpyruvate + reduced 1,1'-carbamoyl methyl viologen anaerobic conditions Proteus vulgaris phenyllactate + oxidized 1,1'-carbamoyl methyl viologen
-
 r
1.1.99.30 phenylpyruvate + reduced benzyl viologen anaerobic conditions Proteus vulgaris phenyllactate + oxidized benzyl viologen
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.99.6 oligomer x * 80000, probably octamer, SDS-PAGE Proteus vulgaris
1.1.99.30 More subunit structure Proteus vulgaris
1.1.99.30 octamer 8 * 80000, SDS-PAGE Proteus vulgaris

Synonyms

EC Number Synonyms Comment Organism
1.1.99.30 HVOR
-
 Proteus vulgaris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.99.30 25
-
 dehydrogenase assay at Proteus vulgaris
1.1.99.30 37
-
 reductase assay at Proteus vulgaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.99.30 8.5
-
 assay at Proteus vulgaris

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.99.6 anthraquinone-2,6-disulfonate artificial electron acceptor Proteus vulgaris
1.1.99.6 molybdopterin mononucleotide
-
 Proteus vulgaris
1.1.99.6 additional information molybenum-containing Fe-S-enzyme without an additional prosthetic group such as flavin or cytochrome c, enzyme contains one molybdenum, four iron and four sulfur atoms per subunit Proteus vulgaris
1.1.99.30 molybdenum cofactor mononucleotide, 1 enzyme molecule contains 1 molybdenum atom Proteus vulgaris
1.1.99.30 additional information enzyme contains iron sulfur clusters, with four iron and 4 sulfur atoms per enzyme molecule, enzyme belongs to the group of molybdoenzymes which possess no further prosthetic group than the iron sulfur clusters Proteus vulgaris

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.99.30 Proteus vulgaris isoelectric focusing
-
 4.9