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Literature summary extracted from

  • Jong, Y.J.; Nelson, M.J.K.; Snell, E.E.
    Enzymes of vitamin B6 degradation. Purification and properties of pyridoxine 5-dehydrogenase (oxidase) (1986), J. Biol. Chem., 261, 15102-15105.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.99.9 4-pyridoxic acid
-
 Arthrobacter sp.
1.1.99.9 Cu2+
-
 Arthrobacter sp.
1.1.99.9 Dithionitrobenzoic acid
-
 Arthrobacter sp.
1.1.99.9 Hg2+
-
 Arthrobacter sp.
1.1.99.9 Isopyridoxal
-
 Arthrobacter sp.
1.1.99.9 N-ethylmaleimide
-
 Arthrobacter sp.
1.1.99.9 Ni2+
-
 Arthrobacter sp.
1.1.99.9 p-chloromercuribenzoate
-
 Arthrobacter sp.
1.1.99.9 pyridoxal
-
 Arthrobacter sp.
1.1.99.9 pyridoxamine
-
 Arthrobacter sp.
1.1.99.9 sulfhydryl reagents
-
 Arthrobacter sp.
1.1.99.9 Zn2+
-
 Arthrobacter sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.9 0.18
-
 pyridoxine
-
 Arthrobacter sp.
1.1.99.9 0.28
-
 O2
-
 Arthrobacter sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.99.9 soluble
-
 Arthrobacter sp.
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.99.9 58600
-
 2 * 58600, SDS-PAGE Arthrobacter sp.
1.1.99.9 112000
-
 gel filtration Arthrobacter sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.99.9 pyridoxine + acceptor Arthrobacter sp. utilization of pyridoxine as sole source of carbon and nitrogen isopyridoxal + reduced acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.9 Arthrobacter sp.
-
 Cr-7, inducible enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.9
-
 Arthrobacter sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.99.9 11.9
-
-
 Arthrobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.9 4-deoxypyridoxine + acceptor 18% of activity with pyridoxine Arthrobacter sp. 3-hydroxy-2-methylpyridine-5-carboxaldehyde + reduced acceptor
-
 ?
1.1.99.9 pyridoxal + acceptor 29% of activity with pyridoxine Arthrobacter sp. 3-hydroxy-2-methylpyridine-4,5-dicarbaldehyde + reduced acceptor
-
 ?
1.1.99.9 pyridoxamine + acceptor 27% of activity with pyridoxine Arthrobacter sp. 4-aminomethyl-5-formyl-2-methylpyridin-3-ol + reduced acceptor
-
 ?
1.1.99.9 pyridoxine + acceptor NAD+, NADP+ Arthrobacter sp. isopyridoxal + reduced acceptor
-
 ?
1.1.99.9 pyridoxine + acceptor O2 or quinones Arthrobacter sp. isopyridoxal + reduced acceptor
-
 ?
1.1.99.9 pyridoxine + acceptor acceptor: 2,6-dichlorophenolindophenol Arthrobacter sp. isopyridoxal + reduced acceptor
-
 ?
1.1.99.9 pyridoxine + acceptor utilization of pyridoxine as sole source of carbon and nitrogen Arthrobacter sp. isopyridoxal + reduced acceptor
-
 ?
1.1.99.9 pyridoxine + O2
-
 Arthrobacter sp. isopyridoxal + H2O2
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.99.9 dimer 2 * 58600, SDS-PAGE Arthrobacter sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.99.9 22
-
 pyridoxine acceptor O2 Arthrobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.99.9 7 8.3 pyridoxine + O2, Tris buffer, phosphate buffer Arthrobacter sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.99.9 6.4 9.5 activity at pH 9.5 in Tris is about 70% of that at pH 7.3 while activities at pH 6.4 and pH 6.0 in phosphate buffer are about 75% and 0% respectively of that at pH 7.3 Arthrobacter sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.99.9 FAD flavoprotein, FAD-dependent Arthrobacter sp.