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Literature summary extracted from

  • Sanchez, M.; Fernandez, J.; Martin, M.; Gibello, A.; Garrido-Pertierra, A.
    Purification and properties of two succinic semialdehyde dehydrogenases from Klebsiella pneumoniae (1989), Biochim. Biophys. Acta, 990, 225-231.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.16 dithiothreitol activator and stabilizer Klebsiella pneumoniae
1.2.1.16 dithiothreitol activation and stabilization, maximum activity at 25 mM Klebsiella pneumoniae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.16 NADPH
-
 Klebsiella pneumoniae
1.2.1.79 additional information not inhibitory: succinate, pyruvate Klebsiella pneumoniae
1.2.1.79 NADPH
-
 Klebsiella pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.16 0.033
-
 NAD+ pH 7.8, 30°C Klebsiella pneumoniae
1.2.1.16 0.04
-
 succinate semialdehyde
-
 Klebsiella pneumoniae
1.2.1.16 0.054
-
 succinate semialdehyde pH 7.8, 30°C Klebsiella pneumoniae
1.2.1.16 0.154
-
 NADP+ pH 7.8, 30°C Klebsiella pneumoniae
1.2.1.16 0.25
-
 NADP+
-
 Klebsiella pneumoniae
1.2.1.79 0.04
-
 succinate semialdehyde pH 7.8, 30°C Klebsiella pneumoniae
1.2.1.79 0.25
-
 NADP+ pH 7.8, 30°C Klebsiella pneumoniae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.16 additional information bivalent kations such as Mg2+, Mn2+, Ca2+ or Fe2+ are not required Klebsiella pneumoniae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.16 59000
-
 4 * 59000, SDS-PAGE Klebsiella pneumoniae
1.2.1.16 275000
-
 gel filtration Klebsiella pneumoniae
1.2.1.16 300000
-
 gel filtration Klebsiella pneumoniae
1.2.1.79 300000
-
 gel filtration Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.16 succinate semialdehyde + NADP+ + H2O Klebsiella pneumoniae implicated in 4-hydroxyphenylacetic acid and gamma-aminobutyric acid catabolism succinate + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.16 Klebsiella pneumoniae
-
-
-
1.2.1.16 Klebsiella pneumoniae
-
 M5a1 strain and mutant M5a18
-
1.2.1.79 Klebsiella pneumoniae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.16 chromatography on Sephacryl S-300, DEAE-Sephacel and Sepharose 4B Klebsiella pneumoniae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.16 0.06
-
 4-hydroxyphenylacetic acid and glucose used as carbon source, NH3 used as N-source Klebsiella pneumoniae
1.2.1.16 0.07
-
 succinate used as carbon source, NH3 used as N-source Klebsiella pneumoniae
1.2.1.16 0.073
-
 nutrient broth used as carbon source, NADP-dependent form Klebsiella pneumoniae
1.2.1.16 0.075
-
 glycerol used as carbon source, NH3 used as N-source Klebsiella pneumoniae
1.2.1.16 0.078
-
 glycerol used as carbon source, 4-aminobutyric acid used as N-source Klebsiella pneumoniae
1.2.1.16 0.08
-
 4-hydroxyphenylacetic acid used as carbon source, NH3 used as N-source Klebsiella pneumoniae
1.2.1.16 0.085
-
 4-aminobutyric acid used as carbon source, NH3 used as N-source Klebsiella pneumoniae
1.2.1.16 0.091
-
 4-hydroxyphenylacetic acid, glucose and cAMP used as carbon source, NH3 used as N-source Klebsiella pneumoniae
1.2.1.16 0.13
-
 4-hydroxyphenylacetic acid and cAMP used as carbon source, NH3 used as N-source Klebsiella pneumoniae
1.2.1.16 8.5
-
 pH 7.8, 30°C Klebsiella pneumoniae
1.2.1.79 4.1
-
 pH 7.8, 30°C Klebsiella pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.16 succinate semialdehyde + NAD+ + H2O
-
 Klebsiella pneumoniae succinate + NADH + 2 H+
-
 ?
1.2.1.16 succinate semialdehyde + NADP+ + H2O
-
 Klebsiella pneumoniae succinate + NADPH + H+
-
 ?
1.2.1.16 succinate semialdehyde + NADP+ + H2O implicated in 4-hydroxyphenylacetic acid and gamma-aminobutyric acid catabolism Klebsiella pneumoniae succinate + NADPH + H+
-
 ?
1.2.1.16 succinate semialdehyde + NADP+ + H2O
-
 Klebsiella pneumoniae succinate + NADPH + 2 H+
-
 ?
1.2.1.79 succinate semialdehyde + NADP+ + H2O
-
 Klebsiella pneumoniae succinate + NADPH + 2 H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.16 tetramer 4 * 59000, SDS-PAGE Klebsiella pneumoniae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.79 30
-
 assay at Klebsiella pneumoniae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.16 7.5 7.8
-
 Klebsiella pneumoniae
1.2.1.79 7.5 7.8
-
 Klebsiella pneumoniae

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.16 NAD+
-
 Klebsiella pneumoniae
1.2.1.16 NADP+ specific for Klebsiella pneumoniae
1.2.1.16 NADP+ 20% of the activity with NAD+ Klebsiella pneumoniae
1.2.1.79 NADP+ specific for NADP+ Klebsiella pneumoniae

Expression

EC Number Organism Comment Expression
1.2.1.16 Klebsiella pneumoniae expression is induced by exogenous succinic semialdehyde additional information