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Literature summary extracted from

  • Yambe, R.; Hukui, T.; Tuboi, S.
    Succinate semialdehyde dehydrogenase of Rhodopseudomonas spheroides which is active with both NADP+ and NAD+ (1971), J. Biochem., 70, 243-247.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.16 sulfhydryl reagents
-
 Cereibacter sphaeroides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.16 0.06
-
 NADP+
-
 Cereibacter sphaeroides
1.2.1.16 0.16 0.18 succinate semialdehyde either with NAD+ or with NADP+ Cereibacter sphaeroides
1.2.1.16 0.8
-
 NAD+
-
 Cereibacter sphaeroides

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.16 Cereibacter sphaeroides
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.16 column chromatography on DEAE-cellulose, Sephadex G-200 and DEAE-Sephadex Cereibacter sphaeroides

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.16 8.5
-
-
 Cereibacter sphaeroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.16 succinate semialdehyde + NAD+ + H2O
-
 Cereibacter sphaeroides succinate + NADH + H+
-
 r
1.2.1.16 succinate semialdehyde + NADP+ + H2O
-
 Cereibacter sphaeroides succinate + NADPH + H+
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.16 9.7
-
 NAD+ Cereibacter sphaeroides
1.2.1.16 9.9
-
 NADP+ Cereibacter sphaeroides

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.16 8.7 10.6 with NAD+, at pH 8.7: 45% of activity maximum, at pH 10.6: about 50% of activity maximum Cereibacter sphaeroides
1.2.1.16 8.7 10.9 with NADP+, at pH 8.7: about 30% of activity maximum, at pH 10.9: about 65% of activity maximum Cereibacter sphaeroides

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.16 NAD+ enzyme is active both with NADP+ and NAD+, Vmax with NAD+ is considerably larger than that obtained with NADP+ Cereibacter sphaeroides
1.2.1.16 NADP+ enzyme is active both with NADP+ and NAD+, Vmax with NAD+ is considerably larger than that obtained with NADP+ Cereibacter sphaeroides