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Literature summary extracted from

  • Testore, G.; Cravanzola, C.; Bedino, S.
    Aldehyde dehydrogenase from rat intestinal mucosa: purification and characterization of an isozyme with high affinity for gamma-aminobutyraldehyde (1999), Int. J. Biochem. Cell Biol., 31, 777-786.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.19 Cationic buffers
-
 Rattus norvegicus
1.2.1.19 ion-exchange resins
-
 Rattus norvegicus
1.2.1.19 NADH
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.19 0.018
-
 4-Aminobutyraldehyde 250 mM phosphate preparation, constant substrate 1 mM NAD+ Rattus norvegicus
1.2.1.19 0.054
-
 acetaldehyde 250 mM phosphate preparation, constant substrate 1 mM NAD+ Rattus norvegicus
1.2.1.19 0.081
-
 4-Aminobutyraldehyde 400 mM phosphate preparation, constant substrate 1 mM NAD+ Rattus norvegicus
1.2.1.19 0.252
-
 Succinic semialdehyde 400 mM phosphate preparation, constant substrate 1 mM NAD+ Rattus norvegicus
1.2.1.19 0.285
-
 Betaine aldehyde 250 mM phosphate preparation, constant substrate 1 mM NAD+ Rattus norvegicus
1.2.1.19 5.428
-
 Succinic semialdehyde 250 mM phosphate preparation, constant substrate 1 mM NAD+ Rattus norvegicus
1.2.1.19 6.9
-
 acetaldehyde 400 mM phosphate preparation, constant substrate 1 mM NAD+ Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.1.19 cytoplasm
-
 Rattus norvegicus 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.19 52000
-
 2 * 52000, SDS-PAGE Rattus norvegicus
1.2.1.19 112000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.19 4-aminobutanal + NAD+ + H2O Rattus norvegicus involved in the metabolism of biogenic amines and in the synthesis of 4-aminobutyric acid 4-aminobutanoate + NADH + H+
-
 ?
1.2.1.19 4-aminobutanal + NAD+ + H2O Rattus norvegicus Wistar- involved in the metabolism of biogenic amines and in the synthesis of 4-aminobutyric acid 4-aminobutanoate + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.19 Rattus norvegicus
-
 Wistar-strain
-
1.2.1.19 Rattus norvegicus Wistar-
-
 Wistar-strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.19 using column chromatography on hydroxylapatite, precipitation with ammonium sulfate, column chromatography on Sephacryl S-300, Phenyl-superose, 5'-AMP-Sepharose and hydroxylapatite Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.19 intestinal mucosa
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.19 additional information
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
1.2.1.19 4°C, 250 mM phosphate, pH 6.8, in the presence of 4 mM dithiothreitol, activity decreases by 20-30% after 7 days, freezing resultes in complete inactivation Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.19 4-aminobutanal + NAD+ + H2O
-
 Rattus norvegicus 4-aminobutanoate + NADH + H+
-
 ?
1.2.1.19 4-aminobutanal + NAD+ + H2O involved in the metabolism of biogenic amines and in the synthesis of 4-aminobutyric acid Rattus norvegicus 4-aminobutanoate + NADH + H+
-
 ?
1.2.1.19 4-aminobutanal + NAD+ + H2O
-
 Rattus norvegicus Wistar- 4-aminobutanoate + NADH + H+
-
 ?
1.2.1.19 4-aminobutanal + NAD+ + H2O involved in the metabolism of biogenic amines and in the synthesis of 4-aminobutyric acid Rattus norvegicus Wistar- 4-aminobutanoate + NADH + H+
-
 ?
1.2.1.19 acetaldehyde + NAD+ + H2O
-
 Rattus norvegicus acetate + NADH + H+
-
 ?
1.2.1.19 acetaldehyde + NAD+ + H2O
-
 Rattus norvegicus Wistar- acetate + NADH + H+
-
 ?
1.2.1.19 betaine aldehyde + NAD+ + H2O
-
 Rattus norvegicus betaine + NADH + H+
-
 ?
1.2.1.19 betaine aldehyde + NAD+ + H2O
-
 Rattus norvegicus Wistar- betaine + NADH + H+
-
 ?
1.2.1.19 succinate semialdehyde + NAD+
-
 Rattus norvegicus succinate + NADH + H+
-
 ?
1.2.1.19 succinate semialdehyde + NAD+
-
 Rattus norvegicus Wistar- succinate + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.19 dimer 2 * 52000, SDS-PAGE Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.19 9 9.5
-
 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.19 NAD+
-
 Rattus norvegicus