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Literature summary extracted from

  • Mueller, U.; Willnow, P.; Ruschig, U.; Hoepner, T.
    Formate dehydrogenase from Pseudomonas oxalaticus (1978), Eur. J. Biochem., 83, 485-498.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.1.9 CN- 0.25 mM Cupriavidus oxalaticus
1.17.1.9 N3- 0.25 mM. Inhibition is partly overcome by addition of formate or NAD+ Cupriavidus oxalaticus
1.17.1.9 NADH inhibition is partly overcome by addition of formate or NAD+ Cupriavidus oxalaticus
1.17.1.9 NO3- 0.25 mM. Inhibition is partly overcome by addition of formate or NAD+ Cupriavidus oxalaticus
1.17.1.9 p-hydroxymercuribenzoate 0.25 mM Cupriavidus oxalaticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.1.9 0.037
-
 2,6-dichlorophenolindophenol formate dehydrogenase I, reaction with formate Cupriavidus oxalaticus
1.17.1.9 0.042
-
 2,6-dichlorophenolindophenol formate dehydrogenase II, reaction with formate Cupriavidus oxalaticus
1.17.1.9 0.105
-
 NAD+ formate dehydrogenase I, reaction with formate Cupriavidus oxalaticus
1.17.1.9 0.11
-
 NAD+ formate dehydrogenase II, reaction with formate Cupriavidus oxalaticus
1.17.1.9 0.13
-
 formate formate dehydrogenase II, reaction with NAD+ Cupriavidus oxalaticus
1.17.1.9 0.135
-
 formate formate dehydrogenase I, reaction with NAD+ Cupriavidus oxalaticus
1.17.1.9 0.2
-
 2,6-dichlorophenolindophenol formate dehydrogenase I or formate dehydrogenase II, reaction with NADH Cupriavidus oxalaticus
1.17.1.9 0.24
-
 benzyl viologen formate dehydrogenase II, reaction with formate Cupriavidus oxalaticus
1.17.1.9 0.25
-
 benzyl viologen formate dehydrogenase I, reaction with formate Cupriavidus oxalaticus
1.17.1.9 0.46
-
 ferricyanide formate dehydrogenase I, reaction with formate Cupriavidus oxalaticus
1.17.1.9 0.5
-
 ferricyanide formate dehydrogenase II, reaction with formate Cupriavidus oxalaticus
1.17.1.9 0.62
-
 ferricyanide formate dehydrogenase II, reaction with NADH Cupriavidus oxalaticus
1.17.1.9 0.69
-
 ferricyanide formate dehydrogenase I, reaction with NADH Cupriavidus oxalaticus
1.17.1.9 2
-
 benzyl viologen formate dehydrogenase II, reaction with NADH Cupriavidus oxalaticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.1.9 59000
-
 1 * 59000 + 1 * 100000, formate dehydrogenase II, SDS-PAGE Cupriavidus oxalaticus
1.17.1.9 59000
-
 2 * 59000 + 2 * 100000, formate dehydrogenase I, SDS-PAGE Cupriavidus oxalaticus
1.17.1.9 100000
-
 1 * 59000 + 1 * 100000, formate dehydrogenase II, SDS-PAGE Cupriavidus oxalaticus
1.17.1.9 100000
-
 2 * 59000 + 2 * 100000, formate dehydrogenase I, SDS-PAGE Cupriavidus oxalaticus
1.17.1.9 175000
-
 formate dehydrogenase II, sucrose density gradient centrifugation Cupriavidus oxalaticus
1.17.1.9 315000
-
 formate dehydrogenase I, sucrose density gradient centrifugation Cupriavidus oxalaticus

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.9 Cupriavidus oxalaticus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.1.9
-
 Cupriavidus oxalaticus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.17.1.9 formate + NAD+ = CO2 + NADH + H+ sequential mechanism Cupriavidus oxalaticus
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.1.9 32
-
-
 Cupriavidus oxalaticus

Storage Stability

EC Number Storage Stability Organism
1.17.1.9 0°C, under anaerobic conditions, stable for some weeks Cupriavidus oxalaticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.9 2 ferricyanide + NADH
-
 Cupriavidus oxalaticus 2 ferrocyanide + NAD+ + H+
-
 ?
1.17.1.9 formate + 2,6-dichloroindophenol
-
 Cupriavidus oxalaticus CO2 + reduced 2,6-dichlorophenolindophenol
-
 ?
1.17.1.9 formate + 2,6-dichlorophenolindophenol
-
 Cupriavidus oxalaticus CO2 + reduced 2,6-dichlorophenolindophenol
-
 r
1.17.1.9 formate + benzyl viologen
-
 Cupriavidus oxalaticus CO2 + reduced benzyl viologen
-
 ?
1.17.1.9 formate + FAD
-
 Cupriavidus oxalaticus CO2 + FADH2
-
 ?
1.17.1.9 formate + ferricyanide
-
 Cupriavidus oxalaticus CO2 + ferrocyanide
-
 ?
1.17.1.9 formate + FMN
-
 Cupriavidus oxalaticus CO2 + FMNH2
-
 ?
1.17.1.9 formate + methyl viologen
-
 Cupriavidus oxalaticus CO2 + reduced methyl viologen
-
 ?
1.17.1.9 formate + methylene blue
-
 Cupriavidus oxalaticus CO2 + reduced methylene blue
-
 ?
1.17.1.9 formate + NAD+
-
 Cupriavidus oxalaticus CO2 + NADH + H+
-
 ?
1.17.1.9 formate + nitrobluetetrazolium
-
 Cupriavidus oxalaticus CO2 + reduced nitrobluetetrazolium
-
 ?
1.17.1.9 formate + phenazine methosulfate
-
 Cupriavidus oxalaticus CO2 + reduced phenazine methosulfate
-
 ?
1.17.1.9 formate + riboflavin
-
 Cupriavidus oxalaticus CO2 + reduced riboflavin
-
 ?
1.17.1.9 NADH + 2,6-dichloroindophenol
-
 Cupriavidus oxalaticus NAD+ + reduced 2,6-dichlorophenolindophenol
-
 ?
1.17.1.9 NADH + benzyl viologen
-
 Cupriavidus oxalaticus NAD+ + reduced benzyl viologen
-
 ?

Subunits

EC Number Subunits Comment Organism
1.17.1.9 dimer 1 * 59000 + 1 * 100000, formate dehydrogenase II, SDS-PAGE Cupriavidus oxalaticus
1.17.1.9 More formate dehydrogenase I is a dimer of formate dehydrogenase II Cupriavidus oxalaticus
1.17.1.9 tetramer 2 * 59000 + 2 * 100000, formate dehydrogenase I, SDS-PAGE Cupriavidus oxalaticus

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.1.9 FMN formate dehydrogenase I contains 1 mol of FMN per mol of enzyme, formate dehydrogenase II contains 2 mol of FMN per mol of enzyme Cupriavidus oxalaticus
1.17.1.9 NAD+
-
 Cupriavidus oxalaticus