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Literature summary extracted from

  • Farinelli, M.P.; Fry, D.W.; Richardson, K.E.
    Isolation, purification, and partial characterization of formate dehydrogenase from soybean seed (1983), Plant Physiol., 73, 858-859.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.1.9 0.0057
-
 NAD+
-
 Glycine soja
1.17.1.9 0.6
-
 formate
-
 Glycine soja

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.1.9 47000
-
 2 * 47000, SDS-PAGE Glycine soja

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.9 Glycine soja
-
 var Beeson
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.1.9
-
 Glycine soja

Reaction

EC Number Reaction Comment Organism Reaction ID
1.17.1.9 formate + NAD+ = CO2 + NADH + H+ ordered mechanism Glycine soja
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.1.9 1.395
-
-
 Glycine soja

Storage Stability

EC Number Storage Stability Organism
1.17.1.9 4°C, 50 mM phosphate buffer, pH 7.5, 1 mM EDTA, 2 mM 2-mercaptoethanol, 9 months, enzyme retains 90 to 98% of its activity Glycine soja

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.9 formate + NAD+
-
 Glycine soja CO2 + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.17.1.9 dimer 2 * 47000, SDS-PAGE Glycine soja

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.17.1.9 50
-
 10 min, stable Glycine soja
1.17.1.9 60
-
 10 min, 88% loss of activity Glycine soja

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.1.9 6
-
-
 Glycine soja

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.17.1.9 5 6 no activity at pH 5.0 or lower, pH 6.0: optimum Glycine soja

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.1.9 NAD+
-
 Glycine soja