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Literature summary extracted from

  • Chang, Y.F.; Adams, E.
    Factors influencing growth on L-lysine by Pseudomonas (1971), Methods Enzymol., 17B, 166-171.
No PubMed abstract available

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.20 170000
-
sucrose density gradient sedimentation, gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.20 glutarate semialdehyde + NAD+ + H2O Pseudomonas putida constitutive levels of the enzyme are produced under all growth conditions. Growth on Lys leads to specific activity levels 2-3fold higher than growth on other amino acids glutarate + NADH
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.20 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.20
-
Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.20 6.5
-
-
Pseudomonas putida

Storage Stability

EC Number Storage Stability Organism
1.2.1.20 0°C, unstable to storage, inclusion of 10% glycerol greatly improves stability Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.20 2-hydroxybutyraldehyde + NAD+ + H2O low activity Pseudomonas putida 2-hydroxybutanoate + NADH
-
?
1.2.1.20 2-oxoglutaric semialdehyde + NAD+ + H2O
-
Pseudomonas putida 2-oxoglutarate + NADH
-
?
1.2.1.20 5-hydroxyvaleraldehyde + NAD+ + H2O low activity Pseudomonas putida 5-hydroxypentanoate + NADH + H+
-
?
1.2.1.20 butyraldehyde + NAD+ + H2O low activity Pseudomonas putida butanoate + NADH
-
?
1.2.1.20 DL-glyceraldehyde + NAD+ + H2O low activity Pseudomonas putida glycerate + NADH
-
?
1.2.1.20 glutaraldehyde + NAD+ + H2O low activity Pseudomonas putida glutarate semialdehyde + NADH
-
?
1.2.1.20 glutarate semialdehyde + NAD+ + H2O
-
Pseudomonas putida glutarate + NADH
-
?
1.2.1.20 glutarate semialdehyde + NAD+ + H2O constitutive levels of the enzyme are produced under all growth conditions. Growth on Lys leads to specific activity levels 2-3fold higher than growth on other amino acids Pseudomonas putida glutarate + NADH
-
?
1.2.1.20 heptaldehyde + NAD+ + H2O low activity Pseudomonas putida heptanoate + NADH
-
?
1.2.1.20 valeraldehyde + NAD+ + H2O low activity Pseudomonas putida valeric acid + NADH + H+
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.20 9
-
-
Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.20 NAD+ coenzyme Pseudomonas putida