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Literature summary extracted from

  • Eaton, R.W.; Chapman, P.J.
    Bacterial metabolism of naphthalene: Construction and use of recombinant bacteria to study ring cleavage of 1,2-dihydroxynaphthalene and subsequent reactions (1992), J. Bacteriol., 174, 7542-7554.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.65 expression in Escherichia coli and Pseudomonas aeruginosa Pseudomonas putida
4.1.2.45 expression in Escherichia coli Pseudomonas putida
5.99.1.4 expression in Escherichia coli Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.2.45 additional information not sensitive to 5 mM EDTA Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.65 salicylaldehyde + NAD+ + H2O Pseudomonas putida
-
 salicylate + NADH
-
 ?
1.2.1.65 salicylaldehyde + NAD+ + H2O Pseudomonas putida PpG1064
-
 salicylate + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.65 Pseudomonas putida
-
-
-
1.2.1.65 Pseudomonas putida PpG1064
-
-
-
4.1.2.45 Pseudomonas putida Q51947
-
-
5.99.1.4 Pseudomonas putida Q51948
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.65 cell culture
-
 Pseudomonas putida
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.2.45 1.55
-
 recombinant enzyme Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.65 salicylaldehyde + NAD+ + H2O
-
 Pseudomonas putida salicylate + NADH
-
 ?
1.2.1.65 salicylaldehyde + NAD+ + H2O
-
 Pseudomonas putida PpG1064 salicylate + NADH
-
 ?
4.1.2.45 (E)-2'-hydroxybenzylidenepyruvate + O2 the equilibrium in this reaction favors cleavage Pseudomonas putida salicylaldehyde + pyruvate
-
 r
4.1.2.45 additional information no activity with trans-benzylidenepyruvate, trans-o-methoxybenzylidenepyruvate or trans-o-hydroxycinnamate. The hydratase-aldolase catalyzes the condensation of pyruvate with several other aromatic aldehydes, including benzaldehyde, to give trans-benzylidenepyruvate. Since benzylidenepyruvate is not a substrate for the enzyme, the reaction is irreversible and can be carried out to completion by using relatively low concentrations of the substrates, benzaldehyde and pyruvate Pseudomonas putida ?
-
 ?
5.99.1.4 2-hydroxy-2H-chromene-2-carboxylate extracts of Escherichia coli JM109 carrying pRE718 catalyze the conversion of trans-o-hydroxybenzylidenepyruvate or 2-hydroxychromene-2-carboxylate to an equilibrium mixture that contained 55% 2-hydroxychromene-2-carboxylate and 45% trans-o-hydroxybenzylidenepyruvate at pH 7. At pH 10 the reaction occus entirely in on direction, the conversion of 2-hydroxychromene-2-carboxylate to trans-o-hydroxybenzylidenepyruvate. The product is identified by nuclear magnetic resonance spectroscopy. This isomerization occurs spontaneously, although at a slower rate than the enzyme-catalyzed reaction Pseudomonas putida (3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate i.e. (E)-2'-hydroxybenzylidenepyruvate, i.e. trans-o-hydroxybenzylidenepyruvate r

Synonyms

EC Number Synonyms Comment Organism
4.1.2.45 tHBPA hydratase-aldolase
-
 Pseudomonas putida
5.99.1.4 2-hydroxychromene-2-carboxylate isomerase
-
 Pseudomonas putida
5.99.1.4 HCCA isomerase
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.65 NAD+
-
 Pseudomonas putida
4.1.2.45 additional information no cofactor required Pseudomonas putida