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Literature summary extracted from

  • Chalmers, R.M.; Scott, A.J.; Fewson, C.A.
    Purification of the benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase encoded by the TOL plasmid pWW53 of Pseudomonas putida MT53 and their preliminary comparison with benzyl alcohol dehydrogenase and benzaldehyde dehydrogenases I and II from Acinetobacter calcoaceticus (1990), J. Gen. Microbiol., 136, 637-643.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.7 NH4+ or K+ or Rb+ required for maximal activity Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.90 0.0406
-
 NAD+ chromosome-encoded enzyme Pseudomonas putida
1.1.1.90 0.081
-
 3-methylbenzyl alcohol plasmid-encoded enzyme Pseudomonas putida
1.1.1.90 0.106
-
 4-methylbenzyl alcohol plasmid-encoded enzyme Pseudomonas putida
1.1.1.90 0.118
-
 4-methylbenzyl alcohol chromosome-encoded enzyme Pseudomonas putida
1.1.1.90 0.121
-
 benzyl alcohol chromosome-encoded enzyme Pseudomonas putida
1.1.1.90 0.146
-
 3-methylbenzyl alcohol chromosome-encoded enzyme Pseudomonas putida
1.1.1.90 0.219
-
 NAD+ plasmid-encoded enzyme Pseudomonas putida
1.1.1.90 0.233
-
 benzyl alcohol plasmid-encoded enzyme Pseudomonas putida
1.1.1.90 0.605
-
 2-Methylbenzyl alcohol plasmid-encoded enzyme Pseudomonas putida
1.1.1.90 0.992
-
 2-Methylbenzyl alcohol chromosome-encoded enzyme Pseudomonas putida
1.2.1.28 0.00038
-
 3-methylbenzaldehyde
-
 Pseudomonas putida
1.2.1.28 0.00097
-
 4-methylbenzaldehyde
-
 Pseudomonas putida
1.2.1.28 0.0012
-
 3-methylbenzaldehyde
-
 Acinetobacter calcoaceticus
1.2.1.28 0.003
-
 4-methylbenzaldehyde
-
 Pseudomonas putida
1.2.1.28 0.003
-
 4-methylbenzaldehyde
-
 Acinetobacter calcoaceticus
1.2.1.28 0.026
-
 2-Methylbenzaldehyde
-
 Pseudomonas putida
1.2.1.28 0.088
-
 2-Methylbenzaldehyde
-
 Acinetobacter calcoaceticus
1.2.1.28 0.33
-
 NAD+
-
 Pseudomonas putida
1.2.1.28 0.46
-
 benzaldehyde
-
 Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.7 K+ or NH4+ or Rb+ required for maximal activity Pseudomonas putida
1.2.1.7 Rb+ or K+ or NH4+ required for maximal activity Pseudomonas putida
1.2.1.28 K+ or NH4+ or Rb+ required for maximal activity, maximum activity occurs at approx. 70 mM K+ Pseudomonas putida
1.2.1.28 K+ or NH4+ or Rb+ required for maximal activity, maximum activity occurs at approx. 70 mM K+ Acinetobacter calcoaceticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.90 43000
-
 4 * 43000, SDS-PAGE Pseudomonas putida
1.1.1.90 82000
-
 gel filtration Pseudomonas putida
1.2.1.7 56300
-
 4 * 56300, SDS-PAGE Pseudomonas putida
1.2.1.7 122000
-
 gel filtration Pseudomonas putida
1.2.1.28 56300
-
 4 * 56300, SDS-PAGE Pseudomonas putida
1.2.1.28 122000 126000 gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.90 benzyl alcohol + NAD+ Pseudomonas putida
-
 benzaldehyde + NADH
-
 ?
1.1.1.90 benzyl alcohol + NAD+ Acinetobacter calcoaceticus
-
 benzaldehyde + NADH
-
 ?
1.1.1.90 benzyl alcohol + NAD+ Pseudomonas putida MT53
-
 benzaldehyde + NADH
-
 ?
1.2.1.7 benzaldehyde + NADP+ + H2O Pseudomonas putida Pseudomonas enzyme on plasmid also active with NAD+, see also EC 1.2.1.28 benzoate + NADPH
-
 ir
1.2.1.7 benzaldehyde + NADP+ + H2O Pseudomonas putida MT53 Pseudomonas enzyme on plasmid also active with NAD+, see also EC 1.2.1.28 benzoate + NADPH
-
 ir
1.2.1.28 benzaldehyde + NAD+ + H2O Pseudomonas putida
-
 benzoate + NADH + H+
-
 ir
1.2.1.28 benzaldehyde + NAD+ + H2O Acinetobacter calcoaceticus
-
 benzoate + NADH + H+
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.90 Acinetobacter calcoaceticus
-
-
-
1.1.1.90 Pseudomonas putida
-
-
-
1.2.1.7 Pseudomonas putida
-
 MT53
-
1.2.1.7 Pseudomonas putida MT53
-
 MT53
-
1.2.1.28 Acinetobacter calcoaceticus
-
-
-
1.2.1.28 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.90 enzyme encoded by plasmid TOL Pseudomonas putida
1.2.1.7
-
 Pseudomonas putida
1.2.1.28
-
 Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.90 376
-
-
 Pseudomonas putida
1.2.1.7 60.2
-
-
 Pseudomonas putida
1.2.1.28 60.2
-
-
 Pseudomonas putida

Storage Stability

EC Number Storage Stability Organism
1.1.1.90 -20°C, concentrated, mixed with ethanediol Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.90 2-methylbenzyl alcohol + NAD+
-
 Pseudomonas putida 2-methylbenzaldehyde + NADH + H+
-
 ?
1.1.1.90 2-methylbenzyl alcohol + NAD+
-
 Pseudomonas putida MT53 2-methylbenzaldehyde + NADH + H+
-
 ?
1.1.1.90 3-methylbenzyl alcohol + NAD+
-
 Pseudomonas putida 3-methylbenzaldehyde + NADH + H+
-
 ?
1.1.1.90 3-methylbenzyl alcohol + NAD+
-
 Pseudomonas putida MT53 3-methylbenzaldehyde + NADH + H+
-
 ?
1.1.1.90 4-methylbenzyl alcohol + NAD+
-
 Pseudomonas putida 4-methylbenzaldehyde + NADH + H+
-
 ?
1.1.1.90 4-methylbenzyl alcohol + NAD+
-
 Pseudomonas putida MT53 4-methylbenzaldehyde + NADH + H+
-
 ?
1.1.1.90 benzyl alcohol + NAD+
-
 Pseudomonas putida benzaldehyde + NADH
-
 ?
1.1.1.90 benzyl alcohol + NAD+
-
 Acinetobacter calcoaceticus benzaldehyde + NADH
-
 ?
1.1.1.90 benzyl alcohol + NAD+
-
 Pseudomonas putida MT53 benzaldehyde + NADH
-
 ?
1.2.1.7 benzaldehyde + NADP+ + H2O Pseudomonas enzyme on plasmid also active with NAD+, see also EC 1.2.1.28 Pseudomonas putida benzoate + NADPH
-
 ir
1.2.1.7 benzaldehyde + NADP+ + H2O Pseudomonas enzyme on plasmid also active with NAD+, see also EC 1.2.1.28 Pseudomonas putida MT53 benzoate + NADPH
-
 ir
1.2.1.28 2-methylbenzaldehyde + NAD+ + H2O
-
 Pseudomonas putida 2-methylbenzoate + NADH
-
 ?
1.2.1.28 2-methylbenzaldehyde + NAD+ + H2O
-
 Acinetobacter calcoaceticus 2-methylbenzoate + NADH
-
 ?
1.2.1.28 3-methylbenzaldehyde + NAD+ + H2O
-
 Pseudomonas putida 3-methylbenzoate + NADH
-
 ?
1.2.1.28 3-methylbenzaldehyde + NAD+ + H2O
-
 Acinetobacter calcoaceticus 3-methylbenzoate + NADH
-
 ?
1.2.1.28 4-methylbenzaldehyde + NAD+ + H2O
-
 Pseudomonas putida 4-methylbenzoate + NADH
-
 ?
1.2.1.28 4-methylbenzaldehyde + NAD+ + H2O
-
 Acinetobacter calcoaceticus 4-methylbenzoate + NADH
-
 ?
1.2.1.28 benzaldehyde + NAD+ + H2O
-
 Pseudomonas putida benzoate + NADH + H+
-
 ir
1.2.1.28 benzaldehyde + NAD+ + H2O
-
 Acinetobacter calcoaceticus benzoate + NADH + H+
-
 ir
1.2.1.28 benzaldehyde + NAD+ + H2O also active with NADP+, see also EC 1.2.1.7 Pseudomonas putida benzoate + NADH + H+
-
 ir
1.2.1.28 benzaldehyde + NAD+ + H2O also active with NADP+, see also EC 1.2.1.7 Acinetobacter calcoaceticus benzoate + NADH + H+
-
 ir

Subunits

EC Number Subunits Comment Organism
1.1.1.90 tetramer
-
 Acinetobacter calcoaceticus
1.1.1.90 tetramer 4 * 43000, SDS-PAGE Pseudomonas putida
1.2.1.7 tetramer 4 * 56300, SDS-PAGE Pseudomonas putida
1.2.1.28 tetramer
-
 Acinetobacter calcoaceticus
1.2.1.28 tetramer 4 * 56300, SDS-PAGE Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.90 9.4
-
-
 Pseudomonas putida
1.2.1.7 9.3
-
-
 Pseudomonas putida
1.2.1.28 9.3
-
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.90 NAD+
-
 Acinetobacter calcoaceticus
1.1.1.90 NAD+ specific for Pseudomonas putida
1.1.1.90 NADH
-
 Pseudomonas putida
1.1.1.90 NADH
-
 Acinetobacter calcoaceticus
1.2.1.7 NAD+ enzyme on Pseudomonas plasmid Pseudomonas putida
1.2.1.7 NADP+
-
 Pseudomonas putida
1.2.1.28 NAD+
-
 Pseudomonas putida
1.2.1.28 NAD+
-
 Acinetobacter calcoaceticus
1.2.1.28 NADP+
-
 Acinetobacter calcoaceticus
1.2.1.28 NADP+ enzyme on Pseudomonas plasmid Pseudomonas putida