Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Whittaker, M.M.; Whittaker, J.W.
    Characterization of recombinant barley oxalate oxidase expressed by Pichia pastoris (2002), J. Biol. Inorg. Chem., 7, 136-145.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.3.4 expressed in Pichia pastoris X33 Hordeum vulgare

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.3.4 fluoride sub mM concentration Hordeum vulgare
1.2.3.4 Iodide sub mM concentration Hordeum vulgare
1.2.3.4 nitrite sub mM concentration Hordeum vulgare

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.3.4 cytosol
-
Hordeum vulgare 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.3.4 Cu2+ 0.015 g per mol protein Hordeum vulgare
1.2.3.4 Fe2+ 0.015 g per mol protein Hordeum vulgare
1.2.3.4 Mn2+ 0.201 g per monomer, 6-coordination Hordeum vulgare
1.2.3.4 Zn2+ 0.36-0.62 g per mol protein Hordeum vulgare

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.3.4 26000
-
alpha6, 6 * 26000, SDS-PAGE Hordeum vulgare
1.2.3.4 140000
-
gel filtration Hordeum vulgare

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.3.4 oxalic acid + O2 + 2 H+ Hordeum vulgare role in plant signaling and defense 2 CO2 + H2O2
-
ir

Organism

EC Number Organism UniProt Comment Textmining
1.2.3.4 Hordeum vulgare
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.2.3.4 by treatment with K3Fe(CN)6 decreases activity, desalting restores initial activity Hordeum vulgare
1.2.3.4 resistant against Na2IrCl6 Hordeum vulgare

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.2.3.4 glycoprotein 3 kDa carbohydrate per 1 mol protein Hordeum vulgare

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.3.4
-
Hordeum vulgare

Storage Stability

EC Number Storage Stability Organism
1.2.3.4 -180°C, several months, no activity loss Hordeum vulgare
1.2.3.4 8°C, several months, no activity loss, conversion in 4 months to 23 kDa protein, loss of carbohydrate Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.3.4 oxalic acid + O2 + 2 H+
-
Hordeum vulgare 2 CO2 + H2O2
-
ir
1.2.3.4 oxalic acid + O2 + 2 H+ role in plant signaling and defense Hordeum vulgare 2 CO2 + H2O2
-
ir

Subunits

EC Number Subunits Comment Organism
1.2.3.4 hexamer alpha6, 6 * 26000, SDS-PAGE Hordeum vulgare

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.3.4 additional information
-
thermally extremely stable Hordeum vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.3.4 4
-
-
Hordeum vulgare