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Literature summary extracted from

  • Koyama, H.
    Purification and characterization of oxalate oxidase from Pseudomonas sp. OX-53 (1988), Agric. Biol. Chem., 52, 743-748.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.3.4 FAD 0.1 mM concentration 66% activation Pseudomonas sp.
1.2.3.4 FMN 0.1 mM concentration 88% activation Pseudomonas sp.
1.2.3.4 hydroquinone 0.1 mM concentration 123% activation Pseudomonas sp.
1.2.3.4 MnSO4 0.1 mM concentration 75% activation Pseudomonas sp.
1.2.3.4 o-phenylenediamine 0.1 mM concentration 505% activation Pseudomonas sp.
1.2.3.4 phenylhydrazine 0.1 mM concentration 280% activation Pseudomonas sp.
1.2.3.4 riboflavin 0.1 mM concentration 111% activation Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.3.4 8-hydroxyquinoline 0.1 mM concentration 41% inhibition Pseudomonas sp.
1.2.3.4 alpha,alpha'-dipyridyl 0.1 mM concentration 31% inhibition Pseudomonas sp.
1.2.3.4 azide 0.1 mM concentration 10% inhibition Pseudomonas sp.
1.2.3.4 CuSO4 0.1 mM concentration 36% inhibition Pseudomonas sp.
1.2.3.4 EDTA 0.1 mM concentration 42% inhibition Pseudomonas sp.
1.2.3.4 HgCl2 0.1 mM 36% inhibition Pseudomonas sp.
1.2.3.4 hydroxylamine 0.1 mM concentration 100% inhibition Pseudomonas sp.
1.2.3.4 iodacetamide 0.1 mM concentration 62% inhibition Pseudomonas sp.
1.2.3.4 KBr 1 mM concentration 58% inhibition Pseudomonas sp.
1.2.3.4 KI 1 mM concentration 94% inhibition Pseudomonas sp.
1.2.3.4 o-phenanthroline 0.1 mM concentration 26% inhibition Pseudomonas sp.
1.2.3.4 Semicarbazide 0.1 mM concentration 57% inhibition Pseudomonas sp.
1.2.3.4 ZnSO4 0.1 mM concentration 12% inhibition Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.3.4 9.5
-
 oxalic acid
-
 Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.3.4 Fe2+ 0.09 atoms per subunit Pseudomonas sp.
1.2.3.4 Mn2+ 1.12 atoms per subunit Pseudomonas sp.
1.2.3.4 Zn2+ 0.36 atoms per subunit Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.3.4 38000
-
 alpha8, 8 * 38000, SDS-PAGE Pseudomonas sp.
1.2.3.4 320000
-
 gel filtration Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
1.2.3.4 Pseudomonas sp.
-
-
-
1.2.3.4 Pseudomonas sp. OX-53
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.3.4 630
-
 O2 consumption Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
1.2.3.4 -20°C, potassium phosphate buffer pH 6.5, 6 months, no activity loss Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.3.4 DL-malic acid + O2
-
 Pseudomonas sp. ?
-
 ?
1.2.3.4 DL-malic acid + O2
-
 Pseudomonas sp. OX-53 ?
-
 ?
1.2.3.4 glyoxylic acid + O2 + 2 H+
-
 Pseudomonas sp. CO2 + formaldehyde + H2O2
-
 ?
1.2.3.4 glyoxylic acid + O2 + 2 H+
-
 Pseudomonas sp. OX-53 CO2 + formaldehyde + H2O2
-
 ?
1.2.3.4 maleic acid + O2
-
 Pseudomonas sp. ?
-
 ?
1.2.3.4 maleic acid + O2
-
 Pseudomonas sp. OX-53 ?
-
 ?
1.2.3.4 oxalic acid + O2 + 2 H+
-
 Pseudomonas sp. 2 CO2 + H2O2
-
 ir
1.2.3.4 oxalic acid + O2 + 2 H+
-
 Pseudomonas sp. OX-53 2 CO2 + H2O2
-
 ir

Subunits

EC Number Subunits Comment Organism
1.2.3.4 octamer alpha8, 8 * 38000, SDS-PAGE Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.3.4 50
-
 after 10 min 100% activity Pseudomonas sp.
1.2.3.4 60
-
 after 10 min, 76% activity Pseudomonas sp.
1.2.3.4 70
-
 after 10 min 9% activity Pseudomonas sp.
1.2.3.4 89
-
 after 3 min 49% activity Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.3.4 4.8
-
-
 Pseudomonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.2.3.4 5.5 7
-
 Pseudomonas sp.