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Literature summary extracted from

  • Jayanthi, C.R.; Madyastha, P.; Madyastha, K.M.
    Microsomal 11alpha-hydroxylation of progesterone in Aspergillus ochraceus: Part I: Characterization of the hydroxylase system (1982), Biochem. Biophys. Res. Commun., 106, 1262-1268.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.99.14 carbon monoxide
-
 Aspergillus ochraceus
1.14.99.14 cytochrome c
-
 Aspergillus ochraceus
1.14.99.14 Metyrapone
-
 Aspergillus ochraceus
1.14.99.14 Metyrapone
-
 Rhizopus stolonifer
1.14.99.14 N-methyl maleimide
-
 Aspergillus ochraceus
1.14.99.14 p-chloromercuribenzoate
-
 Aspergillus ochraceus
1.14.99.14 SKF-525A
-
 Aspergillus ochraceus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.6.2.4 microsome
-
 Aspergillus ochraceus
-
-
1.14.99.14 cytosol
-
 Aspergillus ochraceus 5829
-
1.14.99.14 cytosol
-
 Rhizopus stolonifer 5829
-
1.14.99.14 microsome
-
 Aspergillus ochraceus
-
-
1.14.99.14 microsome
-
 Rhizopus stolonifer
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.99.14 progesterone + NADPH + O2 Rhizopus arrhizus
-
 11alpha-hydroxyprogesterone + NADP+ + H2O
-
 ?
1.14.99.14 progesterone + NADPH + O2 Rhizopus stolonifer
-
 11alpha-hydroxyprogesterone + NADP+ + H2O
-
 ?
1.14.99.14 progesterone + NADPH + O2 Aspergillus ochraceus activity dependent upon NADPH and O2 11alpha-hydroxyprogesterone + NADP+ + H2O
-
 ?
1.14.99.14 progesterone + NADPH + O2 Aspergillus ochraceus NRRL 405 activity dependent upon NADPH and O2 11alpha-hydroxyprogesterone + NADP+ + H2O
-
 ?
1.14.99.14 progesterone + NADPH + O2 Aspergillus ochraceus TS activity dependent upon NADPH and O2 11alpha-hydroxyprogesterone + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.4 Aspergillus ochraceus
-
-
-
1.14.99.14 Aspergillus ochraceus
-
-
-
1.14.99.14 Aspergillus ochraceus NRRL 405
-
-
-
1.14.99.14 Aspergillus ochraceus TS
-
-
-
1.14.99.14 Rhizopus arrhizus
-
-
-
1.14.99.14 Rhizopus stolonifer
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.99.14 mycelium
-
 Aspergillus ochraceus
-
1.14.99.14 mycelium
-
 Rhizopus stolonifer
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.2.4 0.119
-
 105000 x g pellet, microsomes Aspergillus ochraceus
1.14.99.14 0.0484
-
 cytosol Aspergillus ochraceus
1.14.99.14 0.388
-
 microsomes Aspergillus ochraceus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.14 progesterone + NADPH + O2
-
 Rhizopus arrhizus 11alpha-hydroxyprogesterone + NADP+ + H2O
-
 ?
1.14.99.14 progesterone + NADPH + O2
-
 Aspergillus ochraceus 11alpha-hydroxyprogesterone + NADP+ + H2O
-
 ?
1.14.99.14 progesterone + NADPH + O2
-
 Rhizopus stolonifer 11alpha-hydroxyprogesterone + NADP+ + H2O
-
 ?
1.14.99.14 progesterone + NADPH + O2 activity dependent upon NADPH and O2 Aspergillus ochraceus 11alpha-hydroxyprogesterone + NADP+ + H2O
-
 ?
1.14.99.14 progesterone + NADPH + O2
-
 Aspergillus ochraceus NRRL 405 11alpha-hydroxyprogesterone + NADP+ + H2O
-
 ?
1.14.99.14 progesterone + NADPH + O2 activity dependent upon NADPH and O2 Aspergillus ochraceus NRRL 405 11alpha-hydroxyprogesterone + NADP+ + H2O
-
 ?
1.14.99.14 progesterone + NADPH + O2
-
 Aspergillus ochraceus TS 11alpha-hydroxyprogesterone + NADP+ + H2O
-
 ?
1.14.99.14 progesterone + NADPH + O2 activity dependent upon NADPH and O2 Aspergillus ochraceus TS 11alpha-hydroxyprogesterone + NADP+ + H2O
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.6.2.4 7.7
-
-
 Aspergillus ochraceus
1.14.99.14 7.7
-
-
 Aspergillus ochraceus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.99.14 cytochrome P450
-
 Rhizopus arrhizus
1.14.99.14 cytochrome P450
-
 Aspergillus ochraceus
1.14.99.14 cytochrome P450
-
 Rhizopus stolonifer
1.14.99.14 NADH NADH is far less efficient than NADPH Aspergillus ochraceus
1.14.99.14 NADPH
-
 Aspergillus ochraceus