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Literature summary extracted from

  • Crankshaw, D.L.; Hetnarski, K.; Wilkinson, C.F.
    Purification and characterization of NADPH--cytochrome c reductase from the midgut of the southern armyworm (Spodoptera eridania) (1979), Biochem. J., 181, 593-605.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.6.2.4 menadione slight stimulation Spodoptera eridania
1.6.2.4 additional information increasing ionic strength, increase in activity Rattus norvegicus
1.6.2.4 additional information increasing ionic strength, increase in activity Spodoptera eridania

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.2.4 2'-AMP competitive inhibition Spodoptera eridania
1.6.2.4 alizarin
-
 Spodoptera eridania
1.6.2.4 Mersalyl
-
 Spodoptera eridania
1.6.2.4 NADP+ competitive inhibition Spodoptera eridania
1.6.2.4 p-hydroxymercuribenzoate presence of mercaptoethanol or NADPH provides slight protection against inhibition Spodoptera eridania

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.2.4 0.0063
-
 NADPH
-
 Rattus norvegicus
1.6.2.4 0.013
-
 cytochrome c
-
 Rattus norvegicus
1.6.2.4 0.013
-
 cytochrome c
-
 Spodoptera eridania
1.6.2.4 0.0131
-
 cytochrome c
-
 Rattus norvegicus
1.6.2.4 0.0341
-
 NADPH cosubstrate cytochrome c Spodoptera eridania
1.6.2.4 0.0466
-
 cytochrome c cosubstrate NADPH Spodoptera eridania

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.6.2.4 microsome
-
 Rattus norvegicus
-
-
1.6.2.4 microsome
-
 Spodoptera eridania
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.2.4 70000
-
 x * 70000, SDS-PAGE Spodoptera eridania

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.4 Rattus norvegicus
-
 Sprague-Dawley strain
-
1.6.2.4 Rattus norvegicus Sprague-Dawley
-
 Sprague-Dawley strain
-
1.6.2.4 Spodoptera eridania
-
 southern armyworm
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.2.4 column chromatography on Sephadex G-100 and DEAE-cellulose Rattus norvegicus
1.6.2.4 column chromatography on Sephadex G-100 and DEAE-cellulose Spodoptera eridania

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.2.4 liver phenobarbital-treated animals Rattus norvegicus
-
1.6.2.4 midgut of larvae, induction of larvae with pentamethylbenzene Spodoptera eridania
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.2.4 40.5
-
-
 Rattus norvegicus
1.6.2.4 40.5
-
-
 Spodoptera eridania
1.6.2.4 56.1
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
1.6.2.4 -15°C Rattus norvegicus
1.6.2.4 -15°C, more than 1 year Spodoptera eridania
1.6.2.4 0-4°C, several months Spodoptera eridania

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.2.4 2 ferricytochrome c + NADPH additional electron acceptor: ferricyanide Rattus norvegicus 2 ferrocytochrome c + NADP+ + H+
-
 ?
1.6.2.4 2 ferricytochrome c + NADPH additional electron acceptor: ferricyanide Spodoptera eridania 2 ferrocytochrome c + NADP+ + H+
-
 ?
1.6.2.4 2 ferricytochrome c + NADPH additional electron acceptor: neotetrazolium chloride Rattus norvegicus 2 ferrocytochrome c + NADP+ + H+
-
 ?
1.6.2.4 2 ferricytochrome c + NADPH additional electron acceptor: neotetrazolium chloride Spodoptera eridania 2 ferrocytochrome c + NADP+ + H+
-
 ?
1.6.2.4 2 ferricytochrome c + NADPH additional electron acceptor: 2,6-dichlorophenolindophenol Rattus norvegicus 2 ferrocytochrome c + NADP+ + H+
-
 ?
1.6.2.4 2 ferricytochrome c + NADPH additional electron acceptor: 2,6-dichlorophenolindophenol Spodoptera eridania 2 ferrocytochrome c + NADP+ + H+
-
 ?
1.6.2.4 2 ferricytochrome c + NADPH additional electron acceptor: ferricyanide Rattus norvegicus Sprague-Dawley 2 ferrocytochrome c + NADP+ + H+
-
 ?
1.6.2.4 2 ferricytochrome c + NADPH additional electron acceptor: neotetrazolium chloride Rattus norvegicus Sprague-Dawley 2 ferrocytochrome c + NADP+ + H+
-
 ?
1.6.2.4 2 ferricytochrome c + NADPH additional electron acceptor: 2,6-dichlorophenolindophenol Rattus norvegicus Sprague-Dawley 2 ferrocytochrome c + NADP+ + H+
-
 ?
1.6.2.4 ferricytochrome c + NADPH + H+
-
 Spodoptera eridania ferrocytochrome c + NADP+
-
 r

Subunits

EC Number Subunits Comment Organism
1.6.2.4 ?
-
 Rattus norvegicus
1.6.2.4 ? x * 70000, SDS-PAGE Spodoptera eridania

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.6.2.4 36
-
 inactivation above Spodoptera eridania
1.6.2.4 40
-
 50% activity Spodoptera eridania

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.6.2.4 7.5 9
-
 Spodoptera eridania

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.6.2.4 6.5 9
-
 Spodoptera eridania

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.2.4 FAD ratio FAD : FMN 1 : 1 Rattus norvegicus
1.6.2.4 FAD ratio FAD : FMN 1 : 1 Spodoptera eridania
1.6.2.4 FMN ratio FAD : FMN 1 : 1 Rattus norvegicus
1.6.2.4 FMN ratio FAD : FMN 1 : 1 Spodoptera eridania
1.6.2.4 NADPH
-
 Rattus norvegicus
1.6.2.4 NADPH
-
 Spodoptera eridania