Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kubota, S.; Yoshida, Y.; Kumaoka, H.; Furumichi, A.
    Studies on the microsomal electron-transport system of anaerobically grown yeast. V. Purification and characterization of NADPH-cytochrome c reductase (1977), J. Biochem., 81, 197-205.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.6.2.4 FAD, FMN necessary for stabilization during purification Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.2.4 HgCl2 incubation with NADPH provides protection against inhibition Saccharomyces cerevisiae
1.6.2.4 additional information
-
 Saccharomyces cerevisiae
1.6.2.4 p-chloromercuribenzoate incubation with NADPH provides protection against inhibition Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.2.4 0.0034
-
 cytochrome c
-
 Saccharomyces cerevisiae
1.6.2.4 0.0324
-
 NADPH
-
 Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.2.4 70000
-
 gel filtration Saccharomyces cerevisiae
1.6.2.4 72000
-
 1 * 72000, SDS-PAGE Saccharomyces cerevisiae
1.6.2.4 82000 85000 calculation from FAD content Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.6.2.4 additional information Saccharomyces cerevisiae microsomal electron transport system composed of cytochrome b5, cytochrome P450, NADH-cytochrome b5 reductase and NADPH-cytochrome c reductase ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.4 Saccharomyces cerevisiae
-
 grown anaerobically
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.2.4 column chromatography on Sephadex G-150 and DE-52 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.2.4 150 180
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.2.4 2 ferricytochrome c + NADPH additional electron acceptor: ferricyanide Saccharomyces cerevisiae 2 ferrocytochrome c + NADP+ + H+
-
 ?
1.6.2.4 2 ferricytochrome c + NADPH additional electron acceptor: menadione Saccharomyces cerevisiae 2 ferrocytochrome c + NADP+ + H+
-
 ?
1.6.2.4 2 ferricytochrome c + NADPH additional electron acceptor: 2,6-dichlorophenolindophenol Saccharomyces cerevisiae 2 ferrocytochrome c + NADP+ + H+
-
 ?
1.6.2.4 ferricytochrome c + NADPH + H+
-
 Saccharomyces cerevisiae ferrocytochrome c + NADP+
-
 r
1.6.2.4 additional information microsomal electron transport system composed of cytochrome b5, cytochrome P450, NADH-cytochrome b5 reductase and NADPH-cytochrome c reductase Saccharomyces cerevisiae ?
-
 ?
1.6.2.4 NADPH + O2 slow reaction, presence of menadione, or duroquinone, or vitamin K3 essential Saccharomyces cerevisiae NADP+ + O2- superoxide anion ?

Subunits

EC Number Subunits Comment Organism
1.6.2.4 monomer 1 * 72000, SDS-PAGE Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.6.2.4 7.8 8
-
 Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.2.4 FAD tightly bound Saccharomyces cerevisiae
1.6.2.4 FAD ratio FAD : FMN 1 : 1 Saccharomyces cerevisiae
1.6.2.4 FMN ratio FAD : FMN 1 : 1 Saccharomyces cerevisiae
1.6.2.4 FMN loosely bound Saccharomyces cerevisiae
1.6.2.4 NADPH
-
 Saccharomyces cerevisiae