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Literature summary extracted from
- Properties of purified kidney microsomal NADPH-cytochrome c reductase (1974), Arch. Biochem. Biophys., 165, 665-671.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.6.2.4 | unstable during purification | Sus scrofa |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.6.2.4 | NADP+ | competitive inhibition | Sus scrofa |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.6.2.4 | 0.0046 | - |
NADPH | - |
Sus scrofa | |
| 1.6.2.4 | 0.01 | - |
ferricytochrome c | - |
Sus scrofa | |
| 1.6.2.4 | 24 | - |
NADH | - |
Sus scrofa | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.6.2.4 | microsome | - |
Sus scrofa | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.6.2.4 | 65000 | 68000 | gel filtration, kidney | Sus scrofa |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.6.2.4 | Sus scrofa | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.6.2.4 | solubilization with lipase, column chromatography on Sephadex G-100 superfine, DEAE-cellulose and Sephadex G-200 superfine | Sus scrofa |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.6.2.4 | kidney | - |
Sus scrofa | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.6.2.4 | 2 ferricytochrome c + NADPH | additional electron acceptor: neotetrazolium chloride | Sus scrofa | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
| 1.6.2.4 | ferricytochrome c + NADH + H+ | - |
Sus scrofa | ferrocytochrome c + NAD+ | - |
r | |
| 1.6.2.4 | ferricytochrome c + NADPH + H+ | - |
Sus scrofa | ferrocytochrome c + NADP+ | - |
r | |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.6.2.4 | 20.5 | - |
ferricytochrome c | - |
Sus scrofa | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.6.2.4 | FAD | ratio FAD : FMN 1 : 1 | Sus scrofa | |
| 1.6.2.4 | FMN | ratio FAD : FMN 1 : 1 | Sus scrofa | |
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Release 2023.1 (January 2023)
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