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Literature summary extracted from

  • Menting, J.G.T.; Cornish, E.; Scopes, R.K.
    Purification and Partial Characterization of NADPH-cytochrome c reductase from Petunia hybrida flowers (1994), Plant Physiol., 106, 643-650.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.6.2.4 additional information storage of the enzyme at -75°C for 4 months results in a slight activation of the enzyme activity Petunia x hybrida
1.6.2.4 potassium phosphate activity slightly higher than with sodium chloride or sodium sulfate Petunia x hybrida

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.6.2.4 endoplasmic reticulum
-
 Petunia x hybrida 5783
-
1.6.2.4 microsome
-
 Petunia x hybrida
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.2.4 84000
-
 Western blot analysis of fresh microsomal extracts Petunia x hybrida

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.4 Petunia x hybrida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.2.4 using column chromatography on adenosine 2',5'-biphosphate-Sepharose, followed by high-performance anion-exchange chromatography Petunia x hybrida

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.2.4 flower buds and nascent, open flowers Petunia x hybrida
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.2.4 0.02 0.12 flower buds, open flowers exhibits a similar value Petunia x hybrida
1.6.2.4 60 120 purified enzyme Petunia x hybrida

Storage Stability

EC Number Storage Stability Organism
1.6.2.4 -75°C, months, 5 mM 2'-AMP Petunia x hybrida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.2.4 2 ferricytochrome c + NADPH additional electron acceptor: neotetrazolium chloride Petunia x hybrida 2 ferrocytochrome c + NADP+ + H+
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.6.2.4 7.4
-
-
 Petunia x hybrida

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.6.2.4 6 8
-
 Petunia x hybrida

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.2.4 FAD
-
 Petunia x hybrida
1.6.2.4 FMN
-
 Petunia x hybrida
1.6.2.4 NADH activity not detected Petunia x hybrida
1.6.2.4 NADPH
-
 Petunia x hybrida