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Literature summary extracted from
- Oxidation of xenobiotics by plant microsomes, a reconstituted cytochrome P450 system and peroxidase: a comparative study (2000), Phytochemistry, 54, 353-362.
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.6.2.4 | microsome | - |
Tulipa fosteriana | - |
- |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.6.2.4 | Tulipa fosteriana | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.6.2.4 | using column chromatography on DEAE-Sepharose CL 6B and affinity chromatography on a 2',5'-ADP Sepharose 4B column | Tulipa fosteriana |
Renatured (Commentary)
| EC Number | Renatured (Comment) | Organism |
|---|---|---|
| 1.6.2.4 | reconstitution of metabolism of xenobiotics by plant CYPs, reconstituted electron transport chain consisting of partially purified CYP fraction, phospholipid and isolated tulip NADPH : CYP reductase | Tulipa fosteriana |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.6.2.4 | bulb | - |
Tulipa fosteriana | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.6.2.4 | 0.00271 | - |
microsomes | Tulipa fosteriana |
| 1.6.2.4 | 0.0775 | - |
purified enzyme | Tulipa fosteriana |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.6.2.4 | -80°C, 10 mM potassium phosphate, pH 7.4, 0.02% Emulgen 911, 0.002 mM flavinmononucleotide | Tulipa fosteriana |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.6.2.4 | 2 ferricytochrome c + NADPH | - |
Tulipa fosteriana | 2 ferrocytochrome c + NADP+ + H+ | - |
? |  |
| 1.6.2.4 | additional information | oxidation of N-nitrosodimethylamine, N-nitroso-N-methylaniline, aminopyrine and 1-phenylazo-2-naphtol | Tulipa fosteriana | ? | - |
? | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.6.2.4 | NADPH | - |
Tulipa fosteriana | |
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Release 2023.1 (January 2023)
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