EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.140 | Co2+ | 5 mM, 75% inhibition; 75% inhibition at 5 mM Co2+ | Coptis japonica | |
2.1.1.140 | Cu2+ | 47% inhibition at 5 mM Cu2+; 5 mM, 47% inhibition | Coptis japonica | |
2.1.1.140 | Mn2+ | 57% inhibition at 5 mM Mn2+; 5 mM, 57% inhibition | Coptis japonica | |
2.1.1.140 | additional information | no inhibition by PCMB, iodoacetamide; not inhibited by EDTA, iodoacetamide, p-chloromercuribenzoate | Coptis japonica |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.140 | 0.38 | - |
(R,S)-norreticuline | - |
Coptis japonica | |
2.1.1.140 | 0.65 | - |
S-adenosyl-L-methionine | - |
Coptis japonica |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.140 | Ca2+ | 25% increase in activity at 5 mM Ca2+ | Coptis japonica | |
2.1.1.140 | Ca2+ | 5 mM, 25% increase in activity | Coptis japonica | |
2.1.1.140 | additional information | no metal ion requirement | Coptis japonica |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.1.140 | 45000 | - |
4 * 45000, SDS-PAGE | Coptis japonica |
2.1.1.140 | 45000 | - |
alpha, 4 * 45000, SDS-PAGE | Coptis japonica |
2.1.1.140 | 160000 | - |
gel filtration | Coptis japonica |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.140 | (S)-coclaurine + S-adenosyl-L-methionine | Coptis japonica | - |
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.140 | Coptis japonica | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.1.140 | - |
Coptis japonica |
2.1.1.140 | 30-50% ammonium sulfate, Phenyl Sepharose, Q-Sepharose, Mono Q, Mono P, 343fold purification | Coptis japonica |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.1.1.140 | cell culture | - |
Coptis japonica | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.1.1.140 | 0.017 | - |
- |
Coptis japonica |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.140 | (R)-coclaurine + S-adenosyl-L-methionine | - |
Coptis japonica | S-adenosyl-L-homocysteine + (R)-N-methylcoclaurine | - |
? | |
2.1.1.140 | (R,S)-6-O-methylnorlaudanosoline + S-adenosyl-L-methionine | - |
Coptis japonica | S-adenosyl-L-homocysteine + (R,S)-N-methyl-6-O-methyl-norlaudanosoline | - |
? | |
2.1.1.140 | (R,S)-norlaudanosoline + S-adenosyl-L-methionine | - |
Coptis japonica | S-adenosyl-L-homocysteine + (R,S)-N-methylnorlaudanosoline | - |
? | |
2.1.1.140 | (R,S)-norreticuline + S-adenosyl-L-methionine | - |
Coptis japonica | S-adenosyl-L-homocysteine + (R,S)-N-methylnorreticuline | - |
? | |
2.1.1.140 | (S)-coclaurine + S-adenosyl-L-methionine | - |
Coptis japonica | S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine | - |
? | |
2.1.1.140 | 1-methyl-6,7-dimethoxy-1,2,3,4-tetrahydroisoquinoline + S-adenosyl-L-methionine | - |
Coptis japonica | N-methyl-1-methyl-6,7-dimethoxy-1,2,3,4-tetrahydroisoquinoline + S-adenosyl-L-methionine | - |
? | |
2.1.1.140 | 6,7-dimethoxy-1,2,3,4-tetrahydroisoquinoline + S-adenosyl-L-methionine | - |
Coptis japonica | N-methyl-6,7-dimethoxy-1,2,3,4-tetrahydroisoquinoline + S-adenosyl-L-methionine | - |
? | |
2.1.1.140 | additional information | no activity with (R,S)-scoulerine, 1,2,3,4-tetrahydroisoquinoline, 1,2,3,4-tetrahydro-3-isoquinolinecarboxylic acid or (+)-emetine | Coptis japonica | ? | - |
? | |
2.1.1.140 | S-adenosyl-L-methionine + (R)-coclaurine | 122% of the activity with (S)-coclaurine | Coptis japonica | S-adenosyl-L-homocysteine + (R)-N-methylcoclaurine | - |
? | |
2.1.1.140 | S-adenosyl-L-methionine + (R,S)-6-O-methylnorlaudanosoline | 38% of the activity with (S)-coclaurine | Coptis japonica | S-adenosyl-L-homocysteine + (R,S)-N-methyl-6-O-methylnorlaudanosoline | - |
? | |
2.1.1.140 | S-adenosyl-L-methionine + (R,S)-norlaudanosoline | 48% of the activity with (S)-coclaurine | Coptis japonica | S-adenosyl-L-homocysteine + (R,S)-N-methylnorlaudanosoline | - |
? | |
2.1.1.140 | S-adenosyl-L-methionine + (R,S)-norreticuline | 55% of the activity with (S)-coclaurine | Coptis japonica | S-adenosyl-L-homocysteine + (R,S)-N-methylnorreticuline | - |
? | |
2.1.1.140 | S-adenosyl-L-methionine + (S)-coclaurine | sequential bi-substrate mechanism, ordered bi bi or rapid equilibrium random bi bi | Coptis japonica | S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine | - |
? | |
2.1.1.140 | S-adenosyl-L-methionine + 1-methyl-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline | 10% of the activity with (S)-coclaurine | Coptis japonica | S-adenosyl-L-homocysteine + 6,7-dihydroxy-1,N-dimethyl-1,2,3,4-tetrahydroisoquinoline | - |
? | |
2.1.1.140 | S-adenosyl-L-methionine + 6,7-dimethoxy-1,2,3,4-tetrahydroisoquinoline | 39% of the activity with (S)-coclaurine | Coptis japonica | S-adenosyl-L-homocysteine + 6,7-dimethoxy-N-methyl-1,2,3,4-tetrahydroisoquinoline | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.1.1.140 | homotetramer | alpha, 4 * 45000, SDS-PAGE | Coptis japonica |
2.1.1.140 | tetramer | 4 * 45000, SDS-PAGE | Coptis japonica |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.1.140 | CNMT | - |
Coptis japonica |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.1.1.140 | 7 | - |
- |
Coptis japonica |
2.1.1.140 | 7 | - |
50% activity at pH 6.0 and 9.0 | Coptis japonica |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.1.1.140 | 6 | 9 | about 50% of maximal activity at pH 6.0 and 9.0 | Coptis japonica |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
2.1.1.140 | Coptis japonica | chromatofocusing | - |
4.2 |