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Literature summary extracted from

  • Sim, M.K.; Lim, B.C.
    Purification of limonoid glucosyltransferase from navel orange albedo tissue (1997), Phytochemistry, 46, 33-37.
No PubMed abstract available

Application

EC Number Application Comment Organism
2.4.1.210 nutrition limonoid glucosides are important compounds not only for the processing industry but also for the consumer, bitterness due to limonoids is an important economic problem in commercial citrus juice production, limonoid aglycones are converted to nonbitter glucosides by the GTase, enhancement of the limonoid GTase activity through genetic engineering could reduce aglycone concentration, insertion of a gene encoding for GTase into commercial cultivars could create transgenic citrus varieties producing fruits potentially free of limonoid bitterness Citrus sinensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.210 PO43-
-
Fragaria vesca

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.210 Mn2+ 0.05 mM stimulates enzyme activity by 66% over basal activity Citrus sinensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.210 56000
-
Newhall navel orange, SDS-PAGE Citrus sinensis
2.4.1.210 58000
-
Frost navel orange, SDS-PAGE Citrus sinensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.210 limonin + UDP-glucose Citrus sinensis
-
glucosyl-limonin + UDP
-
?
2.4.1.210 limonin + UDP-glucose Citrus unshiu
-
glucosyl-limonin + UDP
-
?
2.4.1.210 limonin + UDP-glucose Citrus aurantium
-
glucosyl-limonin + UDP
-
?
2.4.1.210 limonin + UDP-glucose Fragaria vesca
-
glucosyl-limonin + UDP
-
?
2.4.1.210 limonin + UDP-glucose Citrus maxima
-
glucosyl-limonin + UDP
-
?
2.4.1.210 limonin + UDP-glucose Citrus limonia
-
glucosyl-limonin + UDP
-
?
2.4.1.210 nomilin + UDP-glucose Citrus sinensis
-
UDP + nomilin 17-beta-D-glucopyranoside
-
?
2.4.1.210 nomilin + UDP-glucose Citrus unshiu
-
UDP + nomilin 17-beta-D-glucopyranoside
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.210 Citrus aurantium
-
pommelo hybrid
-
2.4.1.210 Citrus limonia
-
citron
-
2.4.1.210 Citrus maxima
-
pommelo
-
2.4.1.210 Citrus maxima
-
grapefruit, pommelo hybrid Oroblanco
-
2.4.1.210 Citrus sinensis
-
Frost navel orange, Newhall navel orange
-
2.4.1.210 Citrus unshiu
-
mandarin orange
-
2.4.1.210 Fragaria vesca
-
strawberry
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.210
-
Citrus sinensis

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.1.210 UDP-glucose + limonin + H2O = glucosyl-limonin + UDP the enzyme purified from navel orange albedo tissue also acts on the related tetranortriterpenoid nomilin Citrus sinensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.210 albedo
-
Citrus sinensis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.210 0.018
-
-
Citrus sinensis

Storage Stability

EC Number Storage Stability Organism
2.4.1.210 -80°C, 50 mM Tris-HCl, pH 8.0, all acivity is lost Citrus sinensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.210 limonin + UDP-glucose
-
Citrus sinensis glucosyl-limonin + UDP
-
?
2.4.1.210 limonin + UDP-glucose
-
Citrus unshiu glucosyl-limonin + UDP
-
?
2.4.1.210 limonin + UDP-glucose
-
Citrus aurantium glucosyl-limonin + UDP
-
?
2.4.1.210 limonin + UDP-glucose
-
Fragaria vesca glucosyl-limonin + UDP
-
?
2.4.1.210 limonin + UDP-glucose
-
Citrus maxima glucosyl-limonin + UDP
-
?
2.4.1.210 limonin + UDP-glucose
-
Citrus limonia glucosyl-limonin + UDP
-
?
2.4.1.210 nomilin + UDP-glucose
-
Citrus sinensis glucosyl-nomilin + UDP
-
?
2.4.1.210 nomilin + UDP-glucose
-
Citrus sinensis UDP + nomilin 17-beta-D-glucopyranoside
-
?
2.4.1.210 nomilin + UDP-glucose
-
Citrus unshiu UDP + nomilin 17-beta-D-glucopyranoside
-
?

Subunits

EC Number Subunits Comment Organism
2.4.1.210 monomer 56000-58000, SDS-PAGE Citrus sinensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.210 8
-
-
Citrus sinensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.210 6.5 9
-
Citrus sinensis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.210 6.5
-
activity below pH 6.5 is inhibited by a closed D-ring Citrus sinensis