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Literature summary extracted from

  • Lu, W.P.; Kelly, D.P.
    Cellular location and partial purification of the 'thiosulphate-oxidizing enzyme' and the 'trithionate hydrolase' from Thiobacillus tepidarius (1988), J. Gen. Microbiol., 134, 877-885.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.12.1.1 sulfate
-
 Thermithiobacillus tepidarius

General Stability

EC Number General Stability Organism
1.8.2.2 lability of enzyme during purification and storage at -20°C Thermithiobacillus tepidarius

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.2.2 0.004
-
 thiosulfate with cytochrome c as electron acceptor Thermithiobacillus tepidarius
1.8.2.2 0.11
-
 thiosulfate with ferricyanide as electron acceptor Thermithiobacillus tepidarius

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.2.2 periplasm at least 73% of the enzyme activity is recovered in the periplasmic fraction Thermithiobacillus tepidarius
-
-
3.12.1.1 periplasm
-
 Thermithiobacillus tepidarius
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.2.2 45000
-
 3 * 45000, SDS-PAGE Thermithiobacillus tepidarius
1.8.2.2 138000
-
 gel filtration Thermithiobacillus tepidarius

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.2.2 thiosulfate + cytochrome c Thermithiobacillus tepidarius
-
 tetrathionate + reduced cytochrome c
-
 ?
3.12.1.1 trithionate + H2O Thermithiobacillus tepidarius involved in cytochrome c reduction sulfate + thiosulfate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.2.2 Thermithiobacillus tepidarius
-
-
-
3.12.1.1 Thermithiobacillus tepidarius
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.2.2 partial, using ion exchange chromatography, gel filtration and hydrophobic interaction chromatography Thermithiobacillus tepidarius
3.12.1.1 partial Thermithiobacillus tepidarius

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.2.2 1
-
 cytoplasm Thermithiobacillus tepidarius
1.8.2.2 2
-
 membrane Thermithiobacillus tepidarius
1.8.2.2 9.6
-
 periplasm Thermithiobacillus tepidarius
1.8.2.2 316
-
 partially purified enzyme Thermithiobacillus tepidarius
3.12.1.1 0.008 0.014 crude extract Thermithiobacillus tepidarius
3.12.1.1 0.038
-
 partially purified enzyme after stored for 2-3 d at 4°C Thermithiobacillus tepidarius

Storage Stability

EC Number Storage Stability Organism
1.8.2.2 20-30% loss of activity after freezing at -20°C and thawing Thermithiobacillus tepidarius

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.2.2 thiosulfate + cytochrome c
-
 Thermithiobacillus tepidarius tetrathionate + reduced cytochrome c
-
 ?
1.8.2.2 thiosulfate + oxidized acceptor ferricyanide or native cytochrome c act as electron acceptors Thermithiobacillus tepidarius tetrathionate + reduced acceptor
-
 ?
1.8.2.2 thiosulfate + oxidized acceptor cytochrome c acts as electron acceptor Thermithiobacillus tepidarius tetrathionate + reduced acceptor
-
 ?
1.8.2.2 thiosulfate + oxidized acceptor ferricyanide acts as electron acceptor Thermithiobacillus tepidarius tetrathionate + reduced acceptor
-
 ?
3.12.1.1 trithionate + H2O
-
 Thermithiobacillus tepidarius sulfate + thiosulfate
-
 ?
3.12.1.1 trithionate + H2O involved in cytochrome c reduction Thermithiobacillus tepidarius sulfate + thiosulfate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.2.2 trimer 3 * 45000, SDS-PAGE Thermithiobacillus tepidarius