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Literature summary extracted from

  • Rufaut, N.W.; Brennan, S.O.; Hakes, D.J.; Dixon, J.E.; Birch, N.P.
    Purification and characterization of the candidate prohormone-processing enzyme SPC3 produced in a mouse L cell line [published erratum appears in J Biol Chem 1994 Apr 29;269(17):13056] (1993), J. Biol. Chem., 268, 20291-20298.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.75 alpha1-antitrypsin type Pittsburgh as well as wild type Rattus norvegicus
3.4.21.75 antithrombin
-
 Rattus norvegicus
3.4.21.75 EDTA
-
 Rattus norvegicus
3.4.21.75 EGTA
-
 Rattus norvegicus
3.4.21.75 p-hydroxymercuribenzoate
-
 Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.75 Ca2+ required Rattus norvegicus
3.4.21.75 Ca2+ maximal activity at: 10 mM Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.75 additional information Rattus norvegicus probably plays a role in proprotein maturation ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.75 Rattus norvegicus
-
 rat SPC3 cDNA expressed in a mouse L cell line
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.75
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.75 additional information probably plays a role in proprotein maturation Rattus norvegicus ?
-
 ?
3.4.21.75 Protein precursor + H2O circulating human proalbumin variants with a mutation at either of the basic amino acids adjacent to the cleavage site are not cleaved Rattus norvegicus ?
-
 ?
3.4.21.75 Protein precursor + H2O cleavage following the prosequence Arg-Gly-Val-Phe-Arg-Arg Rattus norvegicus ?
-
 ?
3.4.21.75 Protein precursor + H2O also cleaves chicken proalbumin after a single arginine residue following the Arg-Asn-Leu-Gln-Arg-Phe-Ala-Arg prosequence Rattus norvegicus ?
-
 ?
3.4.21.75 Protein precursor + H2O proalbumin (human) Rattus norvegicus ?
-
 ?
3.4.21.75 Pyr-Arg-Thr-Lys-Arg-4-methylcoumarin 7-amide + H2O
-
 Rattus norvegicus ?
-
 ?
3.4.21.75 tert-butoxycarbonyl-Arg-Val-Arg-Arg-4-methylcoumarin 7-amide + H2O
-
 Rattus norvegicus tert-butoxycarbonyl-Arg-Val-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.75 More 3 MW forms: MW 64000, 75000 and 86000, SDS-PAGE, COOH-terminal truncation is responsible for the different molecular mass forms Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.75 6
-
 tert-butyloxycarbonyl-Arg-Val-Arg-Arg-4-methylcoumarin 7-amide Rattus norvegicus