EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.22.32 | alpha2-Macroglobulin | - |
Ananas comosus | |
3.4.22.32 | Human plasma | - |
Ananas comosus | |
3.4.22.32 | additional information | alpha1-antitrypsin | Ananas comosus | |
3.4.22.32 | Rabbit anti-stem bromelain antibodies | - |
Ananas comosus | |
3.4.22.33 | Mercurials | cysteine restores activity; mercaptoethanol restores activity | Ananas comosus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.22.32 | 0.53 | - |
N-Benzyloxycarbonyl-L-Phe-L-Ser methyl ester | - |
Ananas comosus | |
3.4.22.33 | 4 | - |
Nalpha-benzoyl-Arg amide | - |
Ananas comosus | |
3.4.22.33 | 43 | - |
N-benzoyl-L-Arg ethyl ester | - |
Ananas comosus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.22.32 | 33000 | - |
Ananas comosus, determination from sedimentation and diffusion coefficient | Ananas comosus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.22.32 | Ananas comosus | - |
- |
- |
3.4.22.33 | Ananas comosus | - |
- |
- |
EC Number | Oxidation Stability | Organism |
---|---|---|
3.4.22.32 | photosensitized oxidation of stem bromelain in the presence of methylene blue results in partial loss of the enzymatic activity even if the essential sulfhydryl group is protected against oxidation | Ananas comosus |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.22.32 | - |
Ananas comosus |
3.4.22.33 | - |
Ananas comosus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.4.22.32 | stem | - |
Ananas comosus | - |
3.4.22.33 | fruit | - |
Ananas comosus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.22.32 | Benzoyl-L-Arg amide + H2O | - |
Ananas comosus | Benzoyl-L-Arg + NH3 | - |
? | |
3.4.22.32 | Hemoglobin + H2O | denatured | Ananas comosus | Hydrolyzed hemoglobin | - |
? | |
3.4.22.32 | N-Benzyloxycarbonyl-L-Phe-L-Ser methyl ester + H2O | - |
Ananas comosus | ? | - |
? | |
3.4.22.33 | angiotensin II + H2O | cleavage between Tyr4-Ile5 | Ananas comosus | hydrolyzed angiotensin II | cleavage between Tyr4-Ile5 | ? | |
3.4.22.33 | bradykinin + H2O | cleavage between Gly4-Phe5 and Phe5-Ser6 | Ananas comosus | fragments of bradykinin | cleavage between Gly4-Phe5 and Phe5-Ser6 | ? | |
3.4.22.33 | additional information | enzyme catalyzes synthesis of acylamino acid anilides | Ananas comosus | ? | - |
? | |
3.4.22.33 | N-Benzoyl-L-Arg ethyl ester + H2O | - |
Ananas comosus | N-Benzoyl-L-Arg + ethanol | - |
? | |
3.4.22.33 | Nalpha-Benzoyl-Arg amide + H2O | - |
Ananas comosus | Nalpha-Benzoyl-Arg + NH3 | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.22.32 | 3.4 | - |
N-Benzyloxycarbonyl-L-Phe-L-Ser methyl ester | - |
Ananas comosus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.22.32 | 5 | - |
denatured hemoglobin, enzyme form F4 | Ananas comosus |
3.4.22.32 | 5 | 8 | wide plateau, Nalpha-benzoyl-L-Arg amide | Ananas comosus |
3.4.22.33 | 8.3 | - |
casein | Ananas comosus |