Any feedback?
Literature summary extracted from
- Purification and characterization of a proteinase from pineapple fruit, fruit bromelain FA2 (1976), J. Biochem., 79, 1223-1234.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.22.33 | 4 | - |
Nalpha-benzoyl-Arg amide | - |
Ananas comosus |  |
| 3.4.22.33 | 43 | - |
N-benzoyl-L-Arg ethyl ester | - |
Ananas comosus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.33 | 31000 | - |
Ananas comosus, gel electrophoresis | Ananas comosus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.22.33 | Ananas comosus | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.4.22.33 | no glycoprotein | - |
Ananas comosus |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.22.33 | - |
Ananas comosus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.22.33 | fruit | juice | Ananas comosus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.22.33 | angiotensin II + H2O | cleavage between Tyr4-Ile5 | Ananas comosus | hydrolyzed angiotensin II | cleavage between Tyr4-Ile5 | ? | |
| 3.4.22.33 | bradykinin + H2O | cleavage between Gly4-Phe5 and Phe5-Ser6 | Ananas comosus | fragments of bradykinin | cleavage between Gly4-Phe5 and Phe5-Ser6 | ? | |
| 3.4.22.33 | casein + H2O | - |
Ananas comosus | hydrolyzed casein | - |
? | |
| 3.4.22.33 | Hemoglobin + H2O | - |
Ananas comosus | Hydrolyzed hemoglobin | - |
? | |
| 3.4.22.33 | N-Benzoyl-L-Arg ethyl ester + H2O | - |
Ananas comosus | N-Benzoyl-L-Arg + ethanol | - |
? | |
| 3.4.22.33 | Nalpha-Benzoyl-Arg amide + H2O | - |
Ananas comosus | Nalpha-Benzoyl-Arg + NH3 | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.22.33 | monomer | - |
Ananas comosus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.33 | 30 | - |
assay at | Ananas comosus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.33 | additional information | - |
pI: 4.6 | Ananas comosus |
| 3.4.22.33 | 8.3 | - |
casein | Ananas comosus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
