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Literature summary extracted from
- New acid proteases from Scytalidium lignicolum M-133. II. Purification and some enzymatic properties of acid protease A and B of Scytalidium lignicolum ATCC 24568. (1974), Agric. Biol. Chem., 38, 2435-2444.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.23.31 | I2 | - |
Scytalidium lignicola |  |
| 3.4.23.31 | KMnO4 | weak | Scytalidium lignicola | |
| 3.4.23.31 | additional information | a related enzyme from the same organism, proteinase C is also insensitive to these inhibitors; diazoacetyl-DL-norleucine methyl ester; diisopropyl fluorophosphate; methyl 2-diazoacetamidohexanoate, 1,2-epoxy-3-(4-nitrophenyl)propane; not: EDTA; Streptomyces-pepsin inhibitor | Scytalidium lignicola | |
| 3.4.23.31 | N-bromosuccinimide | - |
Scytalidium lignicola | |
| 3.4.23.32 | 1,2-epoxy-3-(4-nitrophenoxy)propane | - |
Scytalidium lignicola | |
| 3.4.23.32 | additional information | diazoacetyl-DL-norleucine methyl ester; diisopropyl fluorophosphate; EDTA; methyl 2-diazoacetamidohexanoate; Streptomyces-pepsin inhibitor (S-PI); sulfhydryl reagents | Scytalidium lignicola | |
| 3.4.23.32 | N-bromosuccinimide | - |
Scytalidium lignicola | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.23.31 | CuCl2 | activates | Scytalidium lignicola | |
| 3.4.23.31 | MnCl2 | activates | Scytalidium lignicola | |
| 3.4.23.32 | CuCl2 | 10 mM, activates | Scytalidium lignicola | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.31 | 43000 | - |
Scytalidium lignicolum, enzyme form A-1 and A-2, gel filtration | Scytalidium lignicola |
| 3.4.23.32 | 22000 | - |
Scytalidium lignicolum, gel filtration | Scytalidium lignicola |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.23.31 | Scytalidium lignicola | - |
A-2 | - |
| 3.4.23.31 | Scytalidium lignicola | - |
enzyme form: A-1 | - |
| 3.4.23.31 | Scytalidium lignicola | - |
ATCC 24568 | - |
| 3.4.23.31 | Scytalidium lignicola A-2 | - |
A-2 | - |
| 3.4.23.32 | Scytalidium lignicola | - |
ATCC 24568 | - |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.4.23.31 | glycoprotein | carbohydrate content: 8-12% (enzyme form A-1 and A-2) | Scytalidium lignicola |
| 3.4.23.32 | additional information | no glycoprotein | Scytalidium lignicola |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.23.31 | enzyme form: A-1 and A-2 | Scytalidium lignicola |
| 3.4.23.32 | - |
Scytalidium lignicola |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.23.31 | culture filtrate | - |
Scytalidium lignicola | - |
| 3.4.23.32 | culture filtrate | - |
Scytalidium lignicola | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.32 | additional information | - |
- |
Scytalidium lignicola |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.23.31 | casein + H2O | - |
Scytalidium lignicola | ? | - |
? | |
| 3.4.23.31 | casein + H2O | - |
Scytalidium lignicola A-2 | ? | - |
? | |
| 3.4.23.32 | casein + H2O | - |
Scytalidium lignicola | hydrolyzed casein | - |
? | |
| 3.4.23.32 | casein + H2O | - |
Scytalidium lignicola A-2 | hydrolyzed casein | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.31 | 55 | - |
enzyme form A-1 and A-2 | Scytalidium lignicola |
| 3.4.23.32 | 65 | - |
- |
Scytalidium lignicola |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.31 | 39 | 63 | 39°C: about 40% of activity maximum, 63°C: 60% of activity maximum | Scytalidium lignicola |
| 3.4.23.32 | 37 | 68 | 37°C: about 30% of activity maximum, 68°C: about 50% of activity maximum | Scytalidium lignicola |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.31 | 37 | - |
pH 2.5-5.5, 20 h, stable | Scytalidium lignicola |
| 3.4.23.31 | 50 | - |
pH 4.0, 15 min, stable below | Scytalidium lignicola |
| 3.4.23.31 | 60 | - |
pH 4.0, 15 min, 60% loss of activity | Scytalidium lignicola |
| 3.4.23.32 | 37 | - |
pH 1.5-5.5, 20 h, stable | Scytalidium lignicola |
| 3.4.23.32 | 60 | - |
pH 4.0, 15 min, stable below | Scytalidium lignicola |
| 3.4.23.32 | 68 | - |
pH 4.0, 15 min, complete loss of activity | Scytalidium lignicola |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.31 | 3 | 3.5 | casein | Scytalidium lignicola |
| 3.4.23.32 | 2 | 2.2 | - |
Scytalidium lignicola |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.31 | 2 | 3.8 | 2: about 50% of activity maximum, 3.8: about 80% of activity maximum | Scytalidium lignicola |
| 3.4.23.32 | 1.3 | 3.5 | 1.3: about 80% of activity maximum, 3.5: about 35% of activity maximum | Scytalidium lignicola |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.31 | 2.5 | 5.5 | 37°C, 20 h, stable | Scytalidium lignicola |
| 3.4.23.31 | 4 | - |
15 min, stable below 50°C, about 60% loss of activity at 60°C | Scytalidium lignicola |
| 3.4.23.32 | 1.5 | 5 | 37°C, 20 h | Scytalidium lignicola |
| 3.4.23.32 | 4 | - |
stable below 60°C, 15 min | Scytalidium lignicola |
| 3.4.23.32 | 4 | - |
15 min, complete loss of activity at 68°C | Scytalidium lignicola |
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