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Literature summary extracted from

  • Sterchi, E.E.; Naim, H.Y.; Lentze, M.J.; Hauri, H.P.; Fransen, J.A.M.
    N-Benzoyl-L-tyrosyl-p-aminobenzoic acid hydrolase: a metalloendopeptidase of the human intestinal microvillus membrane which degrades biologically active peptides (1988), Arch. Biochem. Biophys., 265, 105-118.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.4.24.18 Solubilized PPH is rather labile, immobilization by protein A-Sepharose stabilizes Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.18 1,10-phenanthroline
-
 Homo sapiens
3.4.24.18 2-(N-Hydroxycarboxamido)-4-methylpentanoyl-L-alanyl-glycylamide i.e. Zinkov inhibitor Homo sapiens
3.4.24.18 Ca2+ above 1 mM, metalloproteinase, does not require additional Ca2+ Homo sapiens
3.4.24.18 captopril
-
 Homo sapiens
3.4.24.18 DTT
-
 Homo sapiens
3.4.24.18 EDTA restored by divalent metal ions, Zn2+ being more effective than Ca2+ or Mg2+ Homo sapiens
3.4.24.18 additional information amastatin, bestatin, puromycin, N-tosyl-L-Phe chloromethyl ketone, monoclonal antibody HBB 3/716/36; iodoacetate; leupeptin; no inhibition by phosphoramidon; PMSF, pepstatin Homo sapiens
3.4.24.18 Zn2+ above 1 mM, metalloproteinase, does not require additional Zn2+ Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.18 membrane apical membrane of intestinal epithelial cells Homo sapiens 16020
-
3.4.24.18 microvillus
-
 Homo sapiens 5902
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.18 Calcium metalloendopeptidase Homo sapiens
3.4.24.18 Calcium Ca2+ inhibits above 1 mM Homo sapiens
3.4.24.18 Zinc Zn2+ inhibits above 1 mM Homo sapiens
3.4.24.18 Zinc metalloendopeptidase Homo sapiens
3.4.24.18 Zinc zinc binding motif Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.18 100000
-
 2 * 100000, human, SDS-PAGE in the presence of DTT Homo sapiens
3.4.24.18 200000
-
 human, SDS-PAGE, non-reducing conditions Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.18 Homo sapiens
-
 PPH
-
3.4.24.18 Homo sapiens PPH
-
 PPH
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.24.18 glycoprotein highly glycosylated Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.18 from partially purified microvillar membranes Homo sapiens
3.4.24.18 immunoaffinity chromatography Homo sapiens
3.4.24.18 to near homogeneity Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.18 intestine higher specific activity in distal ileum than in proximal duodenum Homo sapiens
-

Storage Stability

EC Number Storage Stability Organism
3.4.24.18 4°C, immobilized enzyme, 3 months Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.18 angiotensin I + H2O 2 cleavage sites: Tyr-Ile and Phe-His Homo sapiens Asp-Arg-Val-Tyr + Ile-His-Pro-Phe + Ile-His-Pro-Phe-His-Leu
-
 ?
3.4.24.18 angiotensin I + H2O i.e. Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu, cleavage site: Tyr-Ile Homo sapiens Asp-Arg-Val-Tyr + Ile-His-Pro-Phe + Ile-His-Pro-Phe-His-Leu
-
 ?
3.4.24.18 angiotensin II + H2O cleavage site: Tyr-Ile Homo sapiens Asp-Arg-Val-Tyr + Ile-His-Pro-Phe
-
 ?
3.4.24.18 angiotensin II + H2O i.e. Asp-Arg-Val-Tyr-Ile-His-Pro-Phe Homo sapiens Asp-Arg-Val-Tyr + Ile-His-Pro-Phe
-
 ?
3.4.24.18 azocasein + H2O
-
 Homo sapiens fragments of azocasein
-
 ?
3.4.24.18 azocasein + H2O
-
 Homo sapiens PPH fragments of azocasein
-
 ?
3.4.24.18 benzoyl-Gly-His-Leu + H2O very poor substrate, t1/2 of more than 16 h Homo sapiens ?
-
 ?
3.4.24.18 benzoyl-Gly-His-Leu + H2O very poor substrate, t1/2 of more than 16 h Homo sapiens PPH ?
-
 ?
3.4.24.18 bradykinin + H2O i.e. Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg, bradykinin(1-7) and bradykinin(1-8) best substrates next to substance P Homo sapiens Arg-Pro-Pro-Gly + Phe-Ser-Pro-Phe-Arg
-
 ?
3.4.24.18 bradykinin + H2O one cleavage site: Phe-Ser Homo sapiens Arg-Pro-Pro-Gly + Phe-Ser-Pro-Phe-Arg
-
 ?
3.4.24.18 additional information hydrolysis occurs on carboxy side of aromatic residues Homo sapiens ?
-
 ?
3.4.24.18 additional information dansyl-(D)-Ala-Gly-4-phenylalanylglycine, [Leu]-enkephalin, [Met]-enkephalin Homo sapiens ?
-
 ?
3.4.24.18 additional information hydrolysis occurs on carboxy side of aromatic residues Homo sapiens PPH ?
-
 ?
3.4.24.18 additional information dansyl-(D)-Ala-Gly-4-phenylalanylglycine, [Leu]-enkephalin, [Met]-enkephalin Homo sapiens PPH ?
-
 ?
3.4.24.18 N-Benzoyl-L-tyrosyl-4-aminobenzoate + H2O very poor substrate, t1/2 of more than 16 h Homo sapiens N-Benzoyl-L-tyrosine + 4-aminobenzoate
-
 ?
3.4.24.18 N-Benzoyl-L-tyrosyl-4-aminobenzoate + H2O very poor substrate, t1/2 of more than 16 h Homo sapiens PPH N-Benzoyl-L-tyrosine + 4-aminobenzoate
-
 ?
3.4.24.18 Oxytocin + H2O poor substrate Homo sapiens Hydrolyzed oxytocin
-
 ?
3.4.24.18 Substance P + H2O best substrate Homo sapiens Hydrolyzed substance P
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.18 dimer 2 * 100000, human, SDS-PAGE in the presence of DTT Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.18 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.18 additional information
-
 marked microheterogeneity due to glycosylation with pIs in the range of 6-6.85 Homo sapiens
3.4.24.18 7.5 8
-
 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.24.18 7 8 plateau with maximal activity at pH 7.5-8 Homo sapiens